Affinity binding-based system for detecting particulates in a fluid
First Claim
1. An apparatus for detecting a particulate in a fluid, the apparatus comprising a platform comprising a substrate having a surface, wherein said surface defines a detection chamber comprising an area that is coated with a specific binding reagent that specifically binds to the particulate to be detected;
- a fluid sample input means in fluid communication with the detection chamber that is coated with the specific binding reagent;
a wash buffer reservoir containing a wash buffer in fluid communication with the detection chamber that is coated with the specific binding reagent;
a fluid waste receptacle in fluid communication with the detection chamber that is coated with the specific binding reagent;
wherein an amount of a fluid sample comprising a particulate is moved from the fluid sample input means to the detection chamber and incubated thereon for a time sufficient to result in specific binding between the particulate in the fluid sample and the specific binding reagent in the detection chamber; and
wherein the fluid sample is replaced with the wash buffer and displaced into the fluid waste receptacle; and
wherein the wash buffer is further displaced into the fluid waste receptacle; and
wherein a particulate specifically bound to the detection chamber is specifically bound to the specific binding reagent and is detected thereupon.
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Abstract
This invention provides methods an apparatus for detecting and quantifying particulate matter suspended in a fluid. Specifically, the invention provides an integrated, affinity-binding based, analytical system comprising a platform for performing an affinity-binding based assay for specifically binding particulates including microbial cells, and a detection means for detecting the particulates specifically bound to a defined surface or chamber comprising the platform. Methods for using the analytical systems of the invention are also provided.
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Citations
29 Claims
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1. An apparatus for detecting a particulate in a fluid, the apparatus comprising
a platform comprising a substrate having a surface, wherein said surface defines a detection chamber comprising an area that is coated with a specific binding reagent that specifically binds to the particulate to be detected; -
a fluid sample input means in fluid communication with the detection chamber that is coated with the specific binding reagent;
a wash buffer reservoir containing a wash buffer in fluid communication with the detection chamber that is coated with the specific binding reagent;
a fluid waste receptacle in fluid communication with the detection chamber that is coated with the specific binding reagent;
wherein an amount of a fluid sample comprising a particulate is moved from the fluid sample input means to the detection chamber and incubated thereon for a time sufficient to result in specific binding between the particulate in the fluid sample and the specific binding reagent in the detection chamber; and
wherein the fluid sample is replaced with the wash buffer and displaced into the fluid waste receptacle; and
wherein the wash buffer is further displaced into the fluid waste receptacle; and
wherein a particulate specifically bound to the detection chamber is specifically bound to the specific binding reagent and is detected thereupon. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29)
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Specification