Biochips including ion transport detecting structures and methods of use
First Claim
1. A biochip, comprising:
- an array of capillaries, wherein one or more members of said array of capillaries are capable of engaging a particle with a high resistance electrical seal.
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Accused Products
Abstract
The present invention recognizes that the determination of an ion transport function or property using direct detection methods, such as patch-clamps, whole cell recording or single channel recording, are preferable to methods that utilize indirect detection methods, such as FRET based detection system. The present invention provides biochips and methods of use that allow for the direct analysis of ion transport functions or properties using microfabricated structures that can allow for automated detection of one or more ion transport functions or properties. These biochips and methods of use thereof are particularly appropriate for automating the detection of ion transport functions or properties, particularly for screening purposes.
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Citations
11 Claims
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1. A biochip, comprising:
an array of capillaries, wherein one or more members of said array of capillaries are capable of engaging a particle with a high resistance electrical seal. - View Dependent Claims (2, 3, 4, 5)
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6. A method of modifying an ion transport measuring means to enhance the electrical seal of the ion transport measuring means with one or more particles or membranes, comprising:
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a) providing an ion transport measuring means;
b) modifying at least a portion of said ion transport measuring means to have appropriate hydrophilicity or hydrophobicity, texture, or composition to enhance sealing between said ion transport measuring means and said one or more membranes or particles. - View Dependent Claims (7, 8, 9)
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10. The method of claim 10, wherein said modifying comprises baking, fire polishing, or laser polishing.
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11. The method of claim 11, wherein said modifying comprises laser polishing.
Specification