Species specific DNA detection
First Claim
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1. A method for detecting species-specific nucleic acid, comprising:
- a) providing i) a sample selected from the group consisting of a first cell sample from a first species and a cell product derived from said first cell sample, wherein said sample has had previous exposure to second cells from a second species or a cell product derived from said second cells;
ii) first nucleic acid probes specific for nucleic acid derived from said second species;
b) exposing said sample to said first nucleic acid probes under conditions such that said first nucleic acid probes hybridize to said nucleic acid derived from said second species and do not hybridize to nucleic acid from said first cell sample, thereby facilitating the detection of said nucleic acid derived from said second species.
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Abstract
The present invention relates to methods and compositions for the identification of species-specific material in pharmaceutical products. In particular, the present invention relates to methods for the identification of species-specific DNA in a population of a different species or following contact (e.g., growth) with cells of a different species.
67 Citations
38 Claims
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1. A method for detecting species-specific nucleic acid, comprising:
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a) providing i) a sample selected from the group consisting of a first cell sample from a first species and a cell product derived from said first cell sample, wherein said sample has had previous exposure to second cells from a second species or a cell product derived from said second cells;
ii) first nucleic acid probes specific for nucleic acid derived from said second species;
b) exposing said sample to said first nucleic acid probes under conditions such that said first nucleic acid probes hybridize to said nucleic acid derived from said second species and do not hybridize to nucleic acid from said first cell sample, thereby facilitating the detection of said nucleic acid derived from said second species. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. A kit for analyzing a first cell sample from a first species or a cell product derived from said first cell sample, wherein said sample has had previous exposure to cells from a second species or a cell product derived from said cells of said second species comprising:
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a) a container containing first nucleic acid probes specific for nucleic acid derived from said first species;
b) a container containing second nucleic acid probes specific for nucleic acid derived from said second species; and
c) instructions for using said first and second nucleic acid probes for determining the presence or absence of nucleic acid from said second species in said first cell sample. - View Dependent Claims (19, 20, 21, 22, 23, 24, 25, 26)
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27. A method for detecting species-specific nucleic acid, comprising:
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a) providing i) a sample selected from the group consisting of a first cell sample from a first species and a cell product derived from said first cell sample, wherein said sample has had previous exposure to a feeder layer derived from a second species;
ii) first nucleic acid probes specific for nucleic acid derived from said feeder layer from said second species, wherein said first nucleic acid probes are selected from the group consisting of SEQ ID NOs;
1, 2, and 5-26; and
b) exposing said sample to said first nucleic acid probes under conditions such that said first nucleic acid probes hybridize to said nucleic acid derived from said feeder layer and do not hybridize to nucleic acid derived from said sample, thereby facilitating the specific detection of said nucleic acid derived from said second species. - View Dependent Claims (28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38)
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Specification