Multiplexing array techniques

• US 20040146885A1
• Filed: 09/22/2003
• Published: 07/29/2004
• Est. Priority Date: 01/28/2003
• Status: Abandoned Application

• Current Assignee: Nathaniel Tue Tran
• Original Assignee: Nathaniel Tue Tran
• Inventors: Tran, Nathaniel Tue
• Application Number:
  US10/667,882
• Publication Number:
  US 20040146885A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12Q 1/6809 Methods for determination or identification of nucleic acids involving differential detection
• CPC Class Codes: C12Q 2523/125 Bisulfite(s)
• CPC Class Codes: C12Q 2537/143 Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
• CPC Class Codes: C12Q 2565/501 being an array of oligonucleotides
• CPC Class Codes: C40B 30/04 by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
• CPC Class Codes: G01N 33/60 involving radioactive labelled substances

Abstract

A novel method for labeling, detecting and quantifying molecules from multiple samples on the same array is described. The method uses at least one labeled sample to be mixed together with an unlabeled sample and allows competitive binding to an array. The array profiles various molecules from both samples at predetermined locations for detection and quantitative comparison. The signals detected for various molecules from labeled sample will provide indication of presence and relative amount of the same molecules in the unlabeled sample.

Claims

1. A method of analysis comprising the steps of:

(a) labeling at least one sample of test molecules each with a label;

(b) mixing the labeled sample with a second sample into a homogenous mixture of test molecules;

(c) binding said homogeneous mixture to an array; and

(d) detecting at least one sample from said array.

2. The method of analysis of claim 1 further comprising the step of:

detecting and quantifying the quantity of a test molecule in at least one sample.

3. The method of analysis of claim 1 further comprising the step of:

detecting and differentiating between samples of test molecules through an observance of at least one label.

4. The method of analysis of claim 1 further comprising the step of:

comparing relative amount between samples through an observance of at least one label.

5. The method of analysis of claim 1 further comprising the step of:

automated analysis.

6. The method of analysis of claim 1 further comprising the step of:

an observational technique selected from the group consisting of;

post-translational modification of protein study, protein expression study, and gene-expression analysis.

7. The method of analysis of claim 1 wherein the label is a radioactive labeling agent.

8. The method of analysis of claim 1 wherein at least one label is a distinguishing characteristic of the molecule other than an incorporated chemical tag.

9. The method of analysis of claim 1 further comprising the step of:

placing a screen over the array for selective filtering of signal from at least one sample from said array.

10. The method of analysis of claim 1 wherein a sample is labeled by neutron bombardment

16. The method of analysis of claim 1 wherein said array comprising immobilized label.

11. A method of labeling a sample for analysis by neutron bombardment.

12. A method of detection using labeled molecules to detect the present of similar but unlabeled molecules by competitive binding to an array.

13. The method of claim 12 further comprises a step of quantifying said unlabeled molecules.

14. The method of claim 12 wherein the label is a radioactive label.

15. The method of claim 12 wherein said labeled molecules are prepared by neutron bombardment.

17. An array comprising immobilized label.

18. Said array of claim 17 wherein said label includes radioactive isotope.

19. Said array of claim 17 wherein said label includes biotin.

20. Said array of claim 17 wherein said label includes fluorescence.