Tissue collection devices containing biosensors

• US 20040146899A1
• Filed: 10/29/2003
• Published: 07/29/2004
• Est. Priority Date: 12/30/1998
• Status: Abandoned Application

• Current Assignee: Jon Faiz Kayyem
• Original Assignee: Jon Faiz Kayyem
• Inventors: Kayyem, Jon Faiz
• Application Number:
  US10/697,908
• Publication Number:
  US 20040146899A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01N 33/5438 Electrodes

Abstract

The invention relates to tissue collection devices such as blood collection devices that comprise biosensors for the detection of target analytes such as nucleic acids and proteins, including antibodies and enzymes.

Claims

1. A method of detecting a target analyte in a sample comprising:

a) applying an initiation signal to a tissue collection device comprising an electrode comprising;

I) a self-assembled monolayer;

II) an assay complex comprising;

1) a capture binding ligand;

2) said target analyte; and

3) an electron transfer moiety; and

b) detecting electron transfer between said electrode and said electron transfer moiety.

2. A method according to claim 1 wherein said sample is blood.

3. A method according to claim 1 wherein said self-assembled monolayer comprises insulators.

4. A method according to claim 1 wherein said self-assembled monolayer comprises an EFS.

5. A method according to claim 1 wherein said target analyte is nucleic acid.

6. A method according to claim 1 wherein said capture binding ligand is a capture probe.

7. A method according to claim 1 wherein said assay complex comprises a label probe comprising said electron transfer moiety.

8. A method according to claim 1 wherein said electron transfer moiety is ferrocene.