×

Method and system for utilizing somatic cell nuclear transfer embryos as cell donors for additional nuclear transfer

  • US 20040148648A1
  • Filed: 12/09/2003
  • Published: 07/29/2004
  • Est. Priority Date: 12/10/2002
  • Status: Abandoned Application
First Claim
Patent Images

1. A method for cloning a non-human mammal through a nuclear transfer process comprising:

  • (i) obtaining desired differentiated mammalian cells to be used as a source of donor nuclei;

    (ii) obtaining at least one oocyte from a mammal of the same species as the cells which are the source of donor nuclei;

    (iii) enucleating said at least one oocyte;

    (iv) transferring the desired differentiated cell or cell nucleus into the enucleated oocyte;

    (v) simultaneously fusing and activating the cell couplet to form a first transgenic embryo;

    (vi) culturing said first transgenic embryo(es) until it reaches at least the 2-cell developmental stage; and

    (vii) using at least one of the cells of said first transgenic embryo as a donor cell for the production of a transgenic animal through at least a second round of nuclear transfer so as to produce a second transgenic embryo; and

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×