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Novel long-term three-dimensional culture system

  • US 20040151729A1
  • Filed: 11/04/2003
  • Published: 08/05/2004
  • Est. Priority Date: 10/28/2002
  • Status: Abandoned Application
First Claim
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1. A method for generation cultures of small tissues comprising incubating cells from said small tissues in a horizontally-rotating vessel, wherein said vessel (a) contains one or more growth agents that support the growth of said cells and (b) is coated with extracellular matrix protein that promotes cell adhesion under conditions sufficient to allow for proliferation of said cells while retaining the function of said cells.

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