Gastric submucosal tissue as a novel diagnostic tool

• US 20040157283A1
• Filed: 02/10/2004
• Published: 08/12/2004
• Est. Priority Date: 12/10/1996
• Status: Abandoned Application

• Current Assignee: George B. Boder, Stephen F. Badylak
• Original Assignee: George B. Boder, Stephen F. Badylak
• Inventors: Badylak, Stephen F., Boder, George B.
• Application Number:
  US10/775,647
• Publication Number:
  US 20040157283A1
• Time in Patent Office: Days
• US Class Current: 435/34
• CPC Class Codes: C12N 1/20 Bacteria; Culture media therefor
• CPC Class Codes: C12N 2533/92 Amnion; Decellularised dermis or mucosa
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: G01N 33/5091 for testing the pathological state of an organism
• CPC Class Codes: G01N 33/56922 Campylobacter
• CPC Class Codes: Y10S 435/851 Haemophilus

Abstract

A cell culture growth substrate comprising submucosal tissue of a warm-blooded vertebrate and a method for culturing fastidious organisms is described. Submucosal tissue used in accordance with the present invention supports the proliferation of cells when said cells are contacted with submucosal tissue under conditions conducive to cell proliferation.

Claims

1. A method for identifying and diagnosing prokaryotic infectious agents, said method comprising the steps of

obtaining a sample from a source wherein said sample contains prokaryotic infectious agents,

inoculating a cell growth substrate comprising submucosal tissue of a warm-blooded vertebrate with the sample wherein the submucosal tissue is delaminated from muscle layers and the luminal portion of tunica mucosa,

culturing the sample on said substrate under conditions conducive to the proliferation of said prokaryotic infectious agents.

2. The method of claim 1 wherein the sample is taken from a vertebrate species.

3. A method for analyzing, in vitro, the growth characteristics of prokaryotic infectious agents, said method comprising the steps of

inoculating a cell growth substrate comprising submucosal tissue of a warm-blooded vertebrate with the prokaryotic infectious agent wherein the submucosal tissue is delaminated from muscle layers and the luminal portion of tunica mucosa,

culturing the prokaryotic infectious agent on said substrate under a selected variable cell growth condition, and;

histologically examining the prokaryotic infectious agent inoculated on said substrate.