Systemic immune activation method using nucleic acid-lipid complexes
First Claim
1. A method to elicit an immunogen-specific immune response and a systemic, non-specific immune response in a mammal, comprising administering to said mammal a therapeutic composition by a route of administration selected from the group consisting of intravenous and intraperitoneal, said therapeutic composition comprising:
- (a) a liposome delivery vehicle; and
, (b) a recombinant nucleic acid molecule comprising an isolated nucleic acid sequence encoding an immunogen, said nucleic acid sequence being operatively linked to a transcription control sequence.
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Abstract
This invention relates to a method for systemic immune activation which is effective for eliciting both a systemic, non-antigen specific immune response and a strong antigen-specific immune response in a mammal. The method is particularly effective for protecting a mammal from a disease including cancer, a disease associated with allergic inflammation, or an infectious disease. Also disclosed are therapeutic compositions useful in such a method.
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Citations
65 Claims
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1. A method to elicit an immunogen-specific immune response and a systemic, non-specific immune response in a mammal, comprising administering to said mammal a therapeutic composition by a route of administration selected from the group consisting of intravenous and intraperitoneal, said therapeutic composition comprising:
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(a) a liposome delivery vehicle; and
,(b) a recombinant nucleic acid molecule comprising an isolated nucleic acid sequence encoding an immunogen, said nucleic acid sequence being operatively linked to a transcription control sequence. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49)
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50. A method to elicit a tumor antigen-specific immune response and a systemic, non-specific immune response in a mammal that has cancer, comprising administering to a mammal a therapeutic composition by a route of administration selected from the group consisting of intravenous and intraperitoneal administration, said therapeutic composition comprising:
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(a) a liposome delivery vehicle; and
,(b) total RNA isolated from a tumor sample, said RNA encoding tumor antigens. - View Dependent Claims (51, 52, 53)
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54. A method to elicit a pathogen-antigen-specific immune response and a systemic, non-specific immune response in a mammal that has an infectious disease, comprising administering to a mammal a therapeutic composition by a route of administration selected from the group consisting of intravenous and intraperitoneal administration, said therapeutic composition comprising:
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(a) a liposome delivery vehicle; and
,(b) total RNA isolated from an infectious disease pathogen, said RNA encoding pathogen antigens. - View Dependent Claims (55)
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56. A composition for systemic administration to a mammal to elicit an immunogen-specific immune response and a systemic, non-specific immune response, comprising:
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(a) a liposome delivery vehicle; and
(b) a recombinant nucleic acid molecule comprising an isolated nucleic acid sequence encoding an immunogen, said nucleic acid sequence being operatively linked to a transcription control sequence;
wherein said composition has a nucleic acid;
lipid ratio of from about 1;
1 to about 1;
64. - View Dependent Claims (57, 58, 59, 60, 61, 62, 63, 64, 65)
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Specification