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Detection and quantification of aromatic oxygenase genes by real-time PCR

  • US 20040161767A1
  • Filed: 06/30/2003
  • Published: 08/19/2004
  • Est. Priority Date: 06/28/2002
  • Status: Abandoned Application
First Claim
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1. A method for assessing the bioremediation potential of a microbial community in a soil or water sample, comprising:

  • providing a plurality of PCR primer sets, wherein each set corresponds to a distinct family or subfamily of functional aromatic oxygenase genes and is effective to selectively amplify target regions from diverse aromatic oxygenase genes in the corresponding family or subfamily;

    providing a mixture of polynucleotides isolated from microbes present in a soil or water sample;

    performing one or more quantitative PCR amplification reactions using the primer sets to quantify diverse aromatic oxygenase genes of each corresponding family or subfamily in the mixture; and

    determining the bioremediation potential of microbes in the sample based upon results of the one or more quantitative PCR reactions.

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