Sample processing
First Claim
Patent Images
1. A sample processing tubule, comprising:
- at least three segments, each of which is;
defined by the tubule;
fluidly isolated, at least in part by a breakable seal;
so expandable as to receive a volume of fluid expelled from another segment; and
so compressible as to contain substantially no fluid when so compressed;
wherein at least three segments each contains at least one reagent.
3 Assignments
0 Petitions
Accused Products
Abstract
A sample processing tubule may include a first segment, a second segment, and a third segment. Each segment may be defined by the tubule, may be fluidly isolated, at least in part by a breakable seal, may be so expandable as to receive a volume of fluid expelled from another segment, and may be so compressible as to contain substantially no fluid when so compressed. Each segment may contain at least one reagent.
284 Citations
75 Claims
-
1. A sample processing tubule, comprising:
-
at least three segments, each of which is;
defined by the tubule;
fluidly isolated, at least in part by a breakable seal;
so expandable as to receive a volume of fluid expelled from another segment; and
so compressible as to contain substantially no fluid when so compressed;
wherein at least three segments each contains at least one reagent. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45)
-
-
46. A sample processing tubule, comprising:
-
a first segment and a second segment, both of which are;
defined by the tubule;
fluidly isolated by breakable seals;
so expandable as to receive a volume of fluid expelled from another segment; and
so compressible as to contain substantially no fluid when so compressed;
wherein the first segment contains a buffer, and the second segment contains a dry polymerase chain reaction reagent.
-
-
47. A sample processing tubule, comprising:
-
a substantially linear array of contiguous segments, each of which is;
defined by the tubule;
fluidly isolated, at least in part by a breakable seal;
so expandable as to receive a volume of fluid expelled from another segment; and
so compressible as to contain substantially no fluid when so compressed;
wherein;
a segment contains at least one of a lysis reagent and a dilution buffer;
a segment contains at least a nucleic acid binding reagent;
a segment contains at least a wash buffer;
a segment contains at least a nucleic acid eluting regent; and
a segment contains at least nucleic acid amplification reagents.
-
-
48. A method of processing a sample, comprising:
-
introducing a sample into a tubule discretized by breakable seals into a plurality of fluidly isolated segments, wherein the tubule has a proximal end for receiving waste and a distal end for conducting an assay;
incubating the sample in a segment of the tubule with a substance capable of specific binding to a preselected component of the sample;
p1 removing waste from the preselected component by clamping the tubule distally of the segment containing the preselected component and compressing that segment; and
releasing a reagent to mix with the preselected component from a fluidly isolated adjacent distal segment by compressing at least one of the segment containing the preselected component and a segment containing a reagent distal of that segment, thereby opening a breakable seal and either propelling the reagent into the segment containing the preselected component or propelling the preselected component into the segment containing the reagent. - View Dependent Claims (49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75)
-
Specification