Biosensor

• US 20040163953A1
• Filed: 11/07/2003
• Published: 08/26/2004
• Est. Priority Date: 10/06/2000
• Status: Active Grant

• Current Assignee: Roche Diabetes Care Inc. (Roche Holding AG)
• Original Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Inventors: Hill, Brian S., Bhullar, Raghbir S., Wilsey, Christopher D.
• Granted Patent: US 7,287,318 B2
• Time in Patent Office: Days
• US Class Current: 204/403.01
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: Y10T 29/49 Method of mechanical manufacture
• CPC Class Codes: Y10T 29/49002 Electrical device making
• CPC Class Codes: Y10T 29/49007 Indicating transducer
• CPC Class Codes: Y10T 29/49117 Conductor or circuit manufacturing

Abstract

A biosensor is provided that comprises a plate element with a pre-determined reaction zone and a recess positioned adjacent to the reaction zone. The biosensor also comprises a reagent that is positioned on the reaction zone. In preferred embodiments, the recess circumscribes at least a portion of the reaction zone.

Claims

1. A biosensor comprising:

a plate element formed to include a pre-determined reaction zone and a recess positioned adjacent to the reaction zone; and

a reagent being positioned on at least a portion of the reaction zone.

2. The biosensor of claim 1, wherein the plate element includes discrete recesses.

3. The biosensor of claim 2, further comprising electrodes positioned on the reaction zone and the reagent engages at least a portion of the electrodes.

4. The biosensor of claim 3, wherein at least one of the recesses is linear in shape.

5. The biosensor of claim 3, wherein the recesses cooperate with one another to circumscribe at least a portion of the reaction zone.

6. The biosensor of claim 2, wherein at least one recess has a width that is less than 1000 μ

m.

7. The biosensor of claim 2, wherein at least one recess has a width of 1 μ

m to 250 μ

m.

8. The biosensor of claim 2, wherein the electrodes cooperate to define an electrode array with a pre-determined pattern and the recesses are positioned within the pattern.

9. The biosensor of claim 2, wherein at least one of the recesses is curved in shape.

10. The biosensor of claim 9, wherein the recesses cooperate with one another to circumscribe at least a portion of the reaction zone.

11. The biosensor of claim 1, wherein the recess has a width that is less than 1000 μ

m.

12. The biosensor of claim 1, wherein the recess has a width of 1 μ

m to 250 μ

m.

13. The biosenor of claim 1, wherein the recess has height of 0.5 μ

m to 500 μ

m.

14. The biosensor of claim 13, wherein the at least one wall has a height of 8 μ

m to 25 μ

m.

15. The biosensor of claim 1, wherein the recess is rectangular in shape.

23. The biosensor of claim 2, wherein at least one recess has a width of 1 μ

m to 250 μ

m.

16. A biosensor comprising:

a bottom plate element including a first surface formed to include a recess therein;

a reagent positioned on the first surface adjacent to the recess; and

a top plate element coupled to the bottom plate element.

17. The biosensor of claim 16, further comprising an electrode array having a pre-determined electrode pattern positioned between the top and bottom plate elements and the reagent engages at least a portion of the electrode array.

18. The biosensor of claim 17, wherein the biosensor includes two spaced-apart recesses.

19. The biosensor of claim 18, wherein the recesses circumscribe at least a portion of the electrode array.

20. The biosensor of claim 18, wherein the recesses are positioned within the electrode pattern of the array.

21. An electrode set, comprising:

a plate element formed to include a recess therein,

electrodes positioned on the plate element and cooperating to define an electrode array, and

a reagent positioned on at least a portion of the electrodes, wherein the recess circumscribes at least a portion of the electrode array.

22. The electrode set of claim 21, wherein the recess has a width that is less than 1000 μ

m.

24. A method of forming a biosensor, the method comprises the steps of:

providing a plate element;

forming at least one recess in the plate element; and

applying a reagent onto the plate element to define a reaction zone, wherein the at least one recess circumscribes at least a portion of the reaction zone.

25. The method of claim 24, further comprising the step of forming an electrode set in the reaction zone.

26. The method of claim 24, wherein the recesses are formed by laser ablation.