Novel murine polynucleotide sequences and mutant cells and mutant animals defined thereby

• US 20040168209A1
• Filed: 11/04/2003
• Published: 08/26/2004
• Est. Priority Date: 06/12/2000
• Status: Abandoned Application

• Current Assignee: Alejandro Abuin, Arthur T. Sands, Brian Zambrowicz
• Original Assignee: Alejandro Abuin, Arthur T. Sands, Brian Zambrowicz
• Inventors: Sands, Arthur T., Zambrowicz, Brian, Abuin, Alejandro
• Application Number:
  US10/702,075
• Publication Number:
  US 20040168209A1
• Time in Patent Office: Days
• US Class Current: 800/8
• CPC Class Codes: C07H 21/04 with deoxyribosyl as saccharide radical
• CPC Class Codes: C07K 14/47 from mammals
• CPC Class Codes: C12N 2799/027 where the vector is derived from a retrovirus
• CPC Class Codes: C12N 2800/60 Vectors containing traps for, e.g. exons, promoters

Abstract

Novel murine polynucleotides are disclosed that individually identify novel genes into which a retroviral gene trap vector has integrated. Additionally, novel mutated murine ES cells are described that stably incorporate retroviral gene trap constructs into the specifically identified genes. The novel genes and cells thus defined are useful in functional genomic analysis, and in the discovery and development of new therapeutic and diagnostics agents and methods.

Claims

1. An isolated polynucleotide comprising a contiguous stretch of at least about 60 nucleotides first disclosed in at least one of SEQ ID NOS:

1-1,159.

2. An isolated polynucleotide according to claim 1, wherein said polynucleotide sequence comprises at least one of SEQ ID NOS:

1-1,159.

3. An in vitro process for producing an isolated polynucleotide incorporating a sequence capable of hybridizing to a sequence first disclosed in one of SEQ ID NOS:

1-1,159, comprising the steps of;

a) obtaining a polynucleotide template encoding a sequence capable of hybridizing to an GTS of SEQ ID NOS;

1-1,159;

b) contacting said template with a polynucleotide probe comprising at least about 25 contiguous bases first disclosed in SEQ ID NOS;

1-1,159;

c) processing the combined probe and template to allow the specific detection of the combined probe and template; and

d) isolating a clone encoding said template.

4. The process of claim 3 wherein said template is mammalian cDNA.

5. The process of claim 3 wherein said template is mammalian genomic DNA.

6. A process according to claim 4 wherein said template is of human origin.

7. A process for identifying novel polynucleotide sequences comprising the steps of:

a) retrieving a computer readable representation of a polynucleotide sequence first disclosed in at least one of SEQ ID NOS;

1-1,159, or an amino acid sequence encoded thereby, from a computer addressable form of electronic data storage medium;

b) retrieving a computer readable representation of a test polynucleotide or polypeptide sequence from a computer addressable form of electronic data storage medium; and

c) comparing the sequence of said test polynucleotide or polypeptide sequence to a sequence first disclosed in at least one of SEQ ID NOS;

1-1,159, or an amino acid sequence encoded thereby.

8. An isolated murine embryonic stem cell line comprising an engineered retroviral gene trap vector in at least one gene comprising a polynucleotide sequence first disclosed in one of SEQ ID NOS:

1-1,159.

9. The use of an mutated mouse comprising a gene trapped allele from claim 8, to produce a high affinity antibody against a human protein ortholog or homolog of any one of SEQ ID NOS:

1-1,159.