Molecular microarrays and methods for production and use thereof
First Claim
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1. A microarray comprising:
- (a) a substrate, wherein the substrate is derivatized with either;
i) a first compound comprising a first functional group, and at least one layer of a cross-linking compound comprising multiple second functional groups, or ii) a first compound comprising a first functional group, and a polymeric film comprising multiple second functional groups; and
(b) either;
i) a population of at least one entity of interest, wherein the population of at least one entity of interest is associated with a distinct address on the substrate through coupling of the entities and the second functional groups, or ii) a population of microparticles, wherein the population of microparticles has at least one entity of interest coupled thereto, and wherein the population of microparticles is associated with a distinct address on the substrate through coupling of the second functional groups with the microparticles, such that the at least one entity of interest occupies a distinct address on the substrate.
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Abstract
The present invention provides microarrays comprising microparticles with known addresses, wherein the microparticles are coupled to chemical, biological, and/or cellular entities of interest. The invention also provided methods for producing microarrays.
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Citations
53 Claims
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1. A microarray comprising:
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(a) a substrate, wherein the substrate is derivatized with either;
i) a first compound comprising a first functional group, and at least one layer of a cross-linking compound comprising multiple second functional groups, or ii) a first compound comprising a first functional group, and a polymeric film comprising multiple second functional groups; and
(b) either;
i) a population of at least one entity of interest, wherein the population of at least one entity of interest is associated with a distinct address on the substrate through coupling of the entities and the second functional groups, or ii) a population of microparticles, wherein the population of microparticles has at least one entity of interest coupled thereto, and wherein the population of microparticles is associated with a distinct address on the substrate through coupling of the second functional groups with the microparticles, such that the at least one entity of interest occupies a distinct address on the substrate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A microarray comprising:
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(a) a substrate; and
(b) a population of microparticles, wherein the population of microparticles is associated with a distinct address on the substrate, and wherein the population of microparticles has at least one entity of interest coupled thereto, the at least one entity of interest being selected from the group consisting of polypeptides, carbohydrates, cells, hormones, ligands, amino acids, lipids, fatty acids, and small molecules;
such that the at least one entity of interest occupies a distinct address on the substrate. - View Dependent Claims (11, 12, 13, 14)
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15. A microarray comprising:
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(a) a substrate; and
(b) a population of microparticles, wherein each microparticle is less than 1 μ
m in diameter, wherein the population of microparticles is associated with a distinct address on the substrate, and wherein the population of microparticles has at least one entity of interest coupled thereto,such that the at least one entity of interest occupies a distinct address on the substrate. - View Dependent Claims (16, 17, 18, 19, 20)
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21. A microarray produced by a method comprising:
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(a) providing a population of at least one entity of interest, wherein the entities are optionally coupled to microparticles;
(b) providing a substrate, wherein the substrate is derivatized with an activatible compound capable of coupling to the entities of interest or to the optional microparticles;
(c) contacting the population of entities with the substrate; and
(d) activating the activatible compound at the desired location(s) on the substrate, such that the population of entities is coupled to the substrate in the desired location(s). - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30)
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31. A method for constructing a microarray, wherein the method comprises:
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(a) providing a substrate, wherein the substrate is derivatized with either;
i) a first compound comprising a first functional group, and at least one layer of a cross-linking compound comprising multiple second functional groups, or ii) a first compound comprising a first functional group, and a polymeric film comprising multiple second functional groups;
(b) providing either;
i) a population of at least one entity of interest, or ii) a population of microparticles, wherein the population of microparticles has at least one entity of interest coupled thereto;
(c) localizing the population of entities or microparticles to a distinct address on a substrate; and
(d) associating the population of localized entities or microparticles to their distinct address on the substrate through coupling of the second functional groups to the entities of interest or to the microparticles. - View Dependent Claims (32)
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33. A method for constructing a microarray, wherein the method comprises:
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(a) providing a population of at least one entity of interest, wherein the entities are optionally coupled to microparticles;
(b) providing a substrate, wherein the substrate is derivatized with an activatible compound capable of coupling to the entities of interest or to the optional microparticles;
(c) contacting the population of entities with the substrate; and
(d) activating the activatible compound at the desired location(s) on the substrate, such that the population of entities is coupled to the substrate in the desired location(s). - View Dependent Claims (34, 35, 36, 37, 38, 39, 40, 41)
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42. A method of producing microarrays comprising nucleic acid sequences, comprising:
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(a) providing a first microarray comprising;
(i) a first substrate;
(ii) a first population of at least one nucleic acid sequence, wherein the at least one nucleic acid sequence comprises a first nucleic acid hybridization sequence at the distal end of the nucleic acid sequence, wherein the first population of nucleic acid sequence(s) is optionally coupled to microparticle(s), and wherein the population of nucleic acid sequence(s) is associated with a distinct address on the first substrate;
(b) providing a second microarray comprising;
(i) a second substrate;
(ii) a population of second hybridization sequence(s), wherein the second hybridization sequence(s) is complementary to the first hybrization sequence(s), wherein the second population of hybridization sequence(s) are optionally coupled to microparticle(s), and wherein the population of hybridization sequence(s) is associated with a distinct address on the second substrate;
(c) contacting the first and second microarrays under hybridizing conditions, such that the first and second hybridization sequences hybridize;
(d) exposing the hybridized first and second microarrays to nucleotide polymerizing conditions, such that said at least one nucleic acid sequence from the first microarray is used as a template for the production of a complementary nucleic acid sequence on the second microarray. - View Dependent Claims (43, 44, 45)
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46. A method for producing multiple copies of a microarray on a single substrate, wherein the method comprises:
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(a) providing a population of microparticles, wherein the population of microparticles has at least one entity of interest coupled thereto;
(b) providing a substrate for multiple copies of a microarray;
(c) localizing the population of microparticles to the substrate at the desired location(s) for each microarray to be produced on the substrate; and
(d) associating the population of microparticles to the substrate at the desired location(s) for each microarray to be produced on the substrate. - View Dependent Claims (47, 48, 49, 50, 51, 52, 53)
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Specification