Method for in situ, on-chip chemical synthesis
First Claim
1. A method for the synthesis of an array of molecules, comprising:
- (a) providing a substrate having first and second surfaces, and a plurality of isolated porous regions extending through said substrate and communicating with said first and second surfaces;
(b) contacting selected ones of said porous regions with a first reagent;
(c) allowing said first reagent to bind to said selected porous regions; and
(d) withdrawing unreacted said first reagent from said substrate through said selected porous regions by introducing a pressure differential across said substrate.
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Abstract
Apparatus and methods for the synthesis of arrays of molecules bound to a substrate, and in particular oligomeric molecules such as DNA oligonucleotides and peptides. The methods of the invention comprise providing a substrate having first and second surfaces and a plurality of isolated porous regions extending through the substrate and communicating with the first and second surfaces, contacting selected ones of the porous regions with a reagent, allowing the reagent to bind to or otherwise interact with the selected porous regions, and withdrawing unreacted first reagent from the substrate through the selected porous regions by introducing a pressure differential across the substrate. The events may be repeated as required to form oligomeric molecules.
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Citations
62 Claims
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1. A method for the synthesis of an array of molecules, comprising:
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(a) providing a substrate having first and second surfaces, and a plurality of isolated porous regions extending through said substrate and communicating with said first and second surfaces;
(b) contacting selected ones of said porous regions with a first reagent;
(c) allowing said first reagent to bind to said selected porous regions; and
(d) withdrawing unreacted said first reagent from said substrate through said selected porous regions by introducing a pressure differential across said substrate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 21, 22, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61)
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9. A method for the synthesis of an array of oligomeric molecules, comprising:
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(a) providing a substrate having first and second surfaces, and a plurality of isolated porous regions extending through said substrate and communicating with said first and second surfaces;
(b) contacting selected ones of said porous regions with a first reagent including a first monomer;
(c) allowing said first monomer to bind to said selected porous regions;
(d) withdrawing excess said first reagent from said substrate through said selected porous regions;
(e) contacting said selected ones of said porous regions with a second reagent including a second monomer;
(f) allowing said second monomer to couple to said first monomer;
(g) withdrawing excess said second reagent from said substrate through said selected porous regions;
(ii) repeating events (e), (f) and (g) n times using n reagents with n monomers respectively, wherein n equals zero or an integer number, to form said array of oligomeric molecules. - View Dependent Claims (10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 62)
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Specification