Process for preparing dideoxyinosine using adenosine deaminase enzyme
First Claim
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1. A method of making didanosine (ddI) comprising the steps of:
- (a) obtaining an enzyme expressing ddA deaminase activity;
(b) immobilizing the enzyme onto an insoluble support;
(c) contacting the enzyme with a dideoxyadenosine (ddA) solution of at least about 1% weight volume ddA in water for a time and under conditions to produce a ddI solution; and
(d) isolating the ddI from the ddI solution.
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Abstract
A method of making didanosine (ddI) including the steps of: (a) obtaining an enzyme expressing ddA deaminase activity; (b) immobilizing the enzyme onto an insoluble support; (c) contacting the enzyme with a dideoxyadenosine (ddA) solution of at least about 4% weight volume ddA in water for a time and under conditions to produce a ddI solution; and (d) isolating the ddI from the ddI solution. Optionally, the ddI mother liquor is reused in subsequent runs to improve yield.
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Citations
21 Claims
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1. A method of making didanosine (ddI) comprising the steps of:
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(a) obtaining an enzyme expressing ddA deaminase activity;
(b) immobilizing the enzyme onto an insoluble support;
(c) contacting the enzyme with a dideoxyadenosine (ddA) solution of at least about 1% weight volume ddA in water for a time and under conditions to produce a ddI solution; and
(d) isolating the ddI from the ddI solution. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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Specification