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Method and system for comparative genomics for organisms using temperature gradient electrophoresis

  • US 20040178070A1
  • Filed: 07/16/2003
  • Published: 09/16/2004
  • Est. Priority Date: 07/16/2002
  • Status: Abandoned Application
First Claim
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1. A method of determining whether a sequence of a first portion of a polynucleotide of a first organism and a sequence of a first portion of a polynucleotide of a second organism comprise a mismatch, the method comprising:

  • preparing a plurality of duplexes, the duplexes comprising;

    (i) a first polynucleotide strand having a sequence corresponding to a sequence of a first portion of genomic DNA of a first organism; and

    (ii) a first complementary polynucleotide strand having a sequence corresponding to a sequence of a first portion of genomic DNA of a second organism;

    subjecting the duplexes to temperature gradient electrophoresis to obtain first electrophoresis data indicative of the presence of a mismatch between (a) the sequence of the first portion of the genomic DNA of the first organism and (b) the sequence of the complementary portion of the genomic DNA of the second organism.

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