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Compositions and methods for the use of FMOC derivatives in DNA/RNA synthesis

  • US 20040180355A1
  • Filed: 09/24/2003
  • Published: 09/16/2004
  • Est. Priority Date: 09/27/2002
  • Status: Active Grant
First Claim
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1. A method for synthesizing and purifying an oligonucleotide immobilized on a solid support complex, comprising:

  • a) providing;

    i) a polymer support, and ii) four pools of deoxyribonucleoside 3′

    -phosphoramidite monomers in solution, wherein at least one of said monomers contains 9-fluorenylmethoxycarbonyl as an amino protecting group;

    b) sequentially contacting said polymer support with a solution of monomers from one or more of said four pools under conditions such that a sequence of monomers is immobilized on said polymer support to generate an oligonucleotide/polymer support complex, wherein said complex comprises at least one monomer containing 9-fluorenylmethoxycarbonyl and wherein said solution, after said contacting, comprises unreacted material;

    c) treating said complex under conditions such that said unreacted material is substantially removed, thereby creating a purified oligonucleotide/polymer support complex, and d) treating said purified oligonucleotide/polymer support complex under conditions whereby said 9-fluorenylmethoxycarbonyl is released.

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