Surface-enhanced raman nanobiosensor

• US 20040180379A1
• Filed: 08/29/2003
• Published: 09/16/2004
• Est. Priority Date: 08/30/2002
• Status: Abandoned Application

• Current Assignee: Northwestern University
• Original Assignee: Northwestern University
• Inventors: Walsh, Joseph, Haynes, Christy L., Shafer-Peltier, Karen, Van Duyne, Richard P., Glucksberg, Matthew R.
• Application Number:
  US10/652,280
• Publication Number:
  US 20040180379A1
• Time in Patent Office: Days
• US Class Current: 435/7.1
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: G01N 21/658 enhancement Raman, e.g. surface plasmons
• CPC Class Codes: G01N 33/54306 Solid-phase reaction mechanisms

Abstract

The present invention relates to biosensors, in particular to surface-enhanced Raman biosensors for detection of intracellular analytes. In particular, the present invention provides compositions and methods for the in vivo detection of analytes such as glucose.

Claims

1. A composition comprising a plurality of nanobiosensors, said nanobiosensors configured for surface enhanced Raman spectroscopy detection of an analyte.

2. The composition of claim 1, wherein said nanobiosensors are coated with a noble metal.

3. The composition of claim 2, wherein said noble metal comprises silver.

4. The composition of claim 1, wherein said nanobiosensors are configured for quantitative detection of said analyte.

5. The composition of claim 1, wherein said nanobiosensors are configured for use in vivo.

6. The composition of claim 1, wherein said analyte is glucose.

7. The composition of claim 1, wherein said nanobiosensors further comprise a surface bound reversibly-binding receptor, said receptor specific for said analyte.

8. The composition of claim 1, wherein said nanobiosensors further comprise a self-assembled monolayer formed on the surface of said nanobiosensors.

9. A method for detection of an analyte, comprising

a) providing

i) a plurality of nanobiosensors, said nanobiosensors configured for surface enhanced Raman spectroscopy detection of an analyte;

and

ii) a device configured for said surface enhanced Raman spectroscopy detection of said analyte; and

b) contacting said pluarality of nanobiosensors with a bodily fluid comprising said analyte; and

c) detecting a surface enhanced Raman signal from said nanobiosensor using said device.

10. The method of claim 9, wherein the level of said surface enhanced Raman signal is correlated with the concentration of said analyte in said bodily fluid.

11. The method of claim 9, wherein said detecting is in vivo.

12. The method of claim 9, wherein said nanobiosensors are coated with a noble metal.

13. The method of claim 9, wherein said analyte is glucose.

14. The method of claim 9, wherein said nanobiosensors further comprises a surface bound reversibly-binding receptor, said receptor specific for said analyte.

15. The method of claim 9, wherein said nanobiosensors further comprises a self-assembled monolayer formed on the surface of said nanobiosensors.

16. A composition comprising a fiber optic tip coated with a plurality of nanobiosensors, said nanobiosensors configured for surface enhanced Raman spectroscopy detection of glucose.

17. The composition of claim 16, wherein said nanobiosensors are configured for use in vivo.

18. The composition of claim 16, wherein said nanobiosensors further comprise a self-assembled monolayer formed on the surface of said nanobiosensors.

19. The composition of claim 16, wherein said nanobiosensors are configured for quantitative detection of glucose in a physiological concentration range.

20. The composition of claim 16, wherein said nanobiosensors are configured for detection of glucose in the presence of interfering proteins.