Methods and devices for identifying biopolymers using mass spectroscopy
First Claim
1. A method of identifying a biopolymer in a sample that includes one or more biopolymers, the method comprising:
- selecting a mass from a first dataset, wherein said first dataset includes measured masses of the one or more biopolymers;
matching masses in a second dataset with the selected mass, wherein said second dataset includes measured masses of a collection of fragments of the one or more biopolymers, and wherein the matched masses represent fragments of the biopolymer with the selected mass; and
comparing the matched masses to determine a monomer sequence for the biopolymer with the selected mass.
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Abstract
A method of identifying a biopolymer in a sample that includes one or more biopolymers. The biopolymers may be polypeptides, polynucleotides, or polysaccharides. The method makes use of mass spectral datasets. A first dataset includes measured masses of the one or more biopolymers that are in the sample. A second dataset includes measured masses of a collection of fragments of the one or more biopolymers. The method selects a mass from the first dataset and then matches masses from the second dataset with the selected mass. The matched masses represent fragments of the biopolymer with the selected mass. Once the masses in the second dataset have been matched they are compared to determine a monomer sequence for the biopolymer with the selected mass. The method may be repeated with additional masses in the first dataset.
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Citations
39 Claims
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1. A method of identifying a biopolymer in a sample that includes one or more biopolymers, the method comprising:
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selecting a mass from a first dataset, wherein said first dataset includes measured masses of the one or more biopolymers;
matching masses in a second dataset with the selected mass, wherein said second dataset includes measured masses of a collection of fragments of the one or more biopolymers, and wherein the matched masses represent fragments of the biopolymer with the selected mass; and
comparing the matched masses to determine a monomer sequence for the biopolymer with the selected mass. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38)
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39. A computer program product for identifying a biopolymer in a sample that includes one or more biopolymers, comprising a computer readable medium configured with a set of machine executable instructions, the instructions comprising:
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instructions for selecting a mass from a first dataset, wherein said first dataset includes measured masses of the one or more biopolymers;
instructions for matching masses in a second dataset with the selected mass, wherein said second dataset includes measured masses of a collection of fragments of the one or more biopolymers, and wherein the matched masses represent fragments of the biopolymer with the selected mass; and
instructions for comparing the matched masses to determine a monomer sequence for the biopolymer with the selected mass.
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Specification