Determination of pH including hemoglobin correction
First Claim
1. ) A method of determining the pH of a sample, comprising:
- a) Determining an infrared spectrum of the sample;
b) Determining the concentration of hemoglobin of the sample;
c) Selecting a model relating an infrared spectrum to pH that is applicable for samples having the determined hemoglobin concentration;
d) Determining the pH of the sample from the infrared spectrum and the selected model.
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Accused Products
Abstract
Methods and apparatuses of determining the pH of a sample. A method can comprise determining an infrared spectrum of the sample, and determining the hemoglobin concentration of the sample. The hemoglobin concentration and the infrared spectrum can then be used to determine the pH of the sample. In some embodiments, the hemoglobin concentration can be used to select an model relating infrared spectra to pH that is applicable at the determined hemoglobin concentration. In other embodiments, a model relating hemoglobin concentration and infrared spectra to pH can be used. An apparatus according to the present invention can comprise an illumination system, adapted to supply radiation to a sample; a collection system, adapted to collect radiation expressed from the sample responsive to the incident radiation; and an analysis system, adapted to relate information about the incident radiation, the expressed radiation, and the hemoglobin concentration of the sample to pH.
104 Citations
29 Claims
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1. ) A method of determining the pH of a sample, comprising:
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a) Determining an infrared spectrum of the sample;
b) Determining the concentration of hemoglobin of the sample;
c) Selecting a model relating an infrared spectrum to pH that is applicable for samples having the determined hemoglobin concentration;
d) Determining the pH of the sample from the infrared spectrum and the selected model. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. ) A method of determining pH of a sample, comprising:
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a) Determining an infrared spectrum of the sample;
b) Verifying that the spectrum is spectrally consistent with the calibration model;
c) Determining the concentration of hemoglobin of the sample;
d) Determining the pH of the sample from the infrared spectrum, the determined hemoglobin, hematocrit, or equivalent concentration, and a model relating an infrared spectrum and associated hemoglobin concentration to pH. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
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27. ) An apparatus for determining the pH of a sample, comprising:
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a) An illumination system adapted to direct radiation to the sample;
b) A collection system adapted to receive radiation expressed from the sample responsive to incident radiation;
c) An analysis system, comprising a model relating two or more of radiation expressed, incident radiation, and hemoglobin concentration to sample pH. - View Dependent Claims (28)
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29. ) An apparatus for determining the pH of a sample, comprising:
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a) Means for determining an infrared spectrum of the sample;
b) Means for determining the concentration of hemoglobin of the sample;
c) Means for selecting a model relating an infrared spectrum to pH that is applicable for samples having the determined hemoglobin concentration;
d) Means for determining the pH of the sample from the infrared spectrum and the selected model.
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Specification