Releasable polymer arrays

• US 20040185473A1
• Filed: 12/16/2003
• Published: 09/23/2004
• Est. Priority Date: 12/17/2002
• Status: Abandoned Application

• Current Assignee: Affymetrix, Inc. (Thermo Fisher Scientific Incorporated)
• Original Assignee: Affymetrix, Inc. (Thermo Fisher Scientific Incorporated)
• Inventors: McGall, Glenn H., Cuppoletti, Andrea
• Application Number:
  US10/738,381
• Publication Number:
  US 20040185473A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00454 by chemical cleavage from the solid support
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00675 In-situ synthesis on the substrate
• CPC Class Codes: B01J 2219/00711 Light-directed synthesis
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C07H 21/04 with deoxyribosyl as saccharide radical
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/10 Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 50/14 Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
• CPC Class Codes: C40B 80/00 Linkers or spacers specially adapted for combinatorial chemistry or libraries, e.g. traceless linkers or safety-catch linkers

Abstract

Methods are provided for fabricating an array of polymers wherein the polymers may be released from the surface of the array by activation of a releasable group. Also provided are methods for fabricating an array of polymers wherein the polymers can be released from the surface of the array by activation of a releasable group. Arrays of nucleic acids wherein a nucleic acid probe may be released from the array by activation of a releasable groups and methods for fabrication of such arrays are also disclosed.

Claims

1. A method for releasing polymers from an array of polymers comprising the steps of

providing a substrate;

attaching a linker comprising a releasable group to the substrate, wherein said releasable group is labile under a set of conditions;

attaching a first monomer to the linker;

attaching a second monomer to the linker or to the first monomer

repeating said step of attaching a second monomer until a polymer is synthesized; and

releasing said polymer using the set of conditions.

2. The method of claim 1 wherein said monomers are nucleotides.

3. The method of claim 1 wherein said releasable group comprises a photogroup.

4. The method of claim 3 wherein said photogroup is activated by light having a wavelength of 313 nm and below.

5. The method of claim 1 wherein said monomers are amino acids.

6. A releasable polymer array comprising a substrate having a linker comprising a releasable group which is labile under a set of conditions and attached to said linker a polymer, wherein said polymer can be released by exposure of the array to the set of conditions.

7. A releasable polymer array according to claim 6 wherein said polymer is a nucleic acid.

8. A releasable polymer array according to claim 7 wherein said nucleic acid is an oligonucleotide.

9. A releasable polymer array according to claim 6 wherein said releasable group comprises a photogroup.

10. A releasable polymer array according to claim 6 wherein said polymer is a peptide.

11. A nucleic acid array having a releasable nucleic acid probe, said nucleic acid array comprising a substrate having attached thereto a nucleic acid probe, said nucleic acid probe comprising a releasable group which is labile under a set of conditions wherein said releasable group allows release of the probe upon activation.

12. A nucleic acid array according to claim 11 wherein said releasable group comprises a photogroup which may be activated by light having a wavelength of 313 nm and below.

13. A method for fabricating a polymer array having releasable polymers, said method comprising the steps of:

providing a substrate;

attaching a linker to said substrate, said linker comprising a releasable group which is labile under a set of conditions;

reversibly modifying said releasable group with a protecting group to provide a reversibly modified releasable group wherein said modified releasable group is not labile under the set of conditions;

attaching a first monomer to said linker;

attaching a second monomer to said linker or to the first monomer;

repeating said step of attaching said second monomer until a polymer is provided; and

demodifying said reversibly modified releasable group.

14. A method for fabricating a polymer array according to claim 13 wherein said releasable group comprises a photogroup.