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Identification of alleles

  • US 20040185490A1
  • Filed: 03/22/2004
  • Published: 09/23/2004
  • Est. Priority Date: 10/18/1999
  • Status: Abandoned Application
First Claim
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1. A method to determine a nucleotide at a polymorphic locus in a nucleic acid sample, comprising the steps of:

  • amplifying a region of DNA comprising a polymorphic locus in the sample to form amplified DNA products using a primer which terminates at its 3′

    end at the polymorphic locus, wherein the primer comprises a 3′

    portion which is complementary to the region of DNA and a 5′

    portion which is identical in sequence to all or part of a probe on a solid support and not complementary to the region of DNA;

    labeling the amplified DNA products to form labeled amplified DNA products;

    hybridizing the labeled amplified DNA products to the probe on the solid support.

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