ADSORBING PATHOGEN-INACTIVATING COMPOUNDS WITH POROUS PARTICLES IMMOBILIZED IN A MATRIX

• US 20040185553A9
• Filed: 12/07/2001
• Published: 09/23/2004
• Est. Priority Date: 01/06/1998
• Status: Abandoned Application

• Current Assignee: Derek J. Hei
• Original Assignee: Derek J. Hei
• Inventors: Hei, Derek J.
• Application Number:
  US10/011,202
• Publication Number:
  US 20020094568A1
• Time in Patent Office: Days
• US Class Current: 435/308.1
• CPC Class Codes: A01N 1/0205 Chemical aspects
• CPC Class Codes: A01N 1/0236 Mechanical aspects
• CPC Class Codes: A01N 1/0242 Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
• CPC Class Codes: A01N 1/0278 Physical preservation processes
• CPC Class Codes: A61L 2/00 Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for contact lenses A61L12/00; atomisers for disinfecting agents A61M; sterilisation of packages or package contents in association with packaging B65B55/00; treatment of water, waste water, sewage or sludge C02F; disinfecting paper D21H21/36; disinfecting devices for water closets E03D; articles having provision for disinfection, see the relevant subclasses for these articles, e.g. H04R1/12
• CPC Class Codes: A61L 2/0082 using chemical substances
• CPC Class Codes: A61M 1/0209 Multiple bag systems for separating or storing blood components
• CPC Class Codes: A61M 1/0218 with filters
• CPC Class Codes: A61M 1/3679 by absorption A61M1/3675 takes precedence
• CPC Class Codes: B01D 15/00 Separating processes involving the treatment of liquids with solid sorbents using liquid sorbents B01D11/00; ion exchange processes or materials, sorbent materials in general B01J, e.g. sorbents for chromatography B01J20/281; for investigating or analysing materials G01N30/00; Apparatus therefor
• CPC Class Codes: B01J 20/20 comprising free carbon; comprising carbon obtained by carbonising processes
• CPC Class Codes: B01J 20/28004 Sorbent size or size distribution, e.g. particle size
• CPC Class Codes: B01J 20/28019 Spherical, ellipsoidal or cylindrical
• CPC Class Codes: B01J 20/28026 Particles within, immobilised, dispersed, entrapped in or on a matrix, e.g. a resin
• CPC Class Codes: B01J 20/28028 Particles immobilised within fibres or filaments
• CPC Class Codes: B01J 20/28057 Surface area, e.g. B.E.T specific surface area
• CPC Class Codes: B01J 20/28078 Pore diameter
• CPC Class Codes: C07D 219/10 attached in position 9
• CPC Class Codes: C07D 405/12 linked by a chain containing hetero atoms as chain links
• CPC Class Codes: C07D 493/04 Ortho-condensed systems
• CPC Class Codes: C07F 9/64 : Acridine or hydrogenated acridine ring systems

Abstract

Methods and devices are provided for reducing the concentration of low molecular weight compounds in a biological composition containing cells while substantially maintaining a desired biological activity of the biological composition. The device comprises highly porous adsorbent particles, and the adsorbent particles are immobilized by an inert matrix.

Claims

1. A device for reducing the concentration of a low molecular weight compound in a biological composition containing cells, wherein the device comprises an inert matrix containing highly adsorbent particles, and wherein the diameter of the adsorbent particles ranges from about 100 μ

m to about 1500 μ

m, and wherein the cells in the biological composition treated with the device substantially maintain their desired biological activity, and wherein the device is for use in a batch process.

2. A device according to claim 1 further comprising a container that is compatible with the biological composition.

3. A device according to claim 1, wherein the adsorbent particles have a surface area greater than about 750 m²/g.

4. A device according to claim 3, wherein the adsorbent particles have a surface area between about 1000 m²/g and 3000 m²/g.

5. A device according to claim 1, wherein the adsorbent particles are polyaromatic adsorbent particles.

6. A device according to claim 5, wherein the polyaromatic adsorbent particles possess superior wetting properties.

7. A device according to claim 5, wherein the polyaromatic adsorbent particles comprise a hypercrosslinked polystyrene network.

8. A device according to claim 1, wherein the adsorbent particles are activated carbon particles.

9. A device according to claim 8, wherein the activated carbon particles are derived from a synthetic source.

10. A device according to claim 9, wherein the low molecular weight compound is a quencher.

11. A device according to claim 9, wherein the activated carbon particles are roughly spherical and are of a diameter ranging from about 300 μ

m to about 900 μ

m.

12. A device selected from devices according to claim 1, 5 or 8, wherein the inert matrix is a synthetic polymer fiber.

13. A device according to claim 12, wherein the synthetic polymeric fiber comprises a polymer core with a high melting temperature surrounded by a sheath with a lower melting temperature.

14. A device according to claim 12, wherein the inert matrix is a particulate network.

15. A device according to claim 14, wherein the particulate network comprises polyethylene particles.

16. A device according to claim 1, wherein the adsorbent particles are immobilized in the matrix.

17. A device according to claim 1, wherein the low molecular weight compound is a nucleic acid targeting agent or a photosensitizer.

18. A device according to claim 1, wherein the low molecular weight compound is an acridine derivative, a psoralen derivative or a dye.

19. A device according to claim 1, wherein the low molecular weight compound is a biological response modifier.

20. A device according to claim 19, wherein the biological response modifier is activated complement.

21. A device according to claim 1, wherein the low molecular weight compound is a quencher.

22. A device according to claim 1, wherein the low molecular weight compound is a polyamine derivative.

23. A device according to claim 1, wherein the desired biological activity maintained by the cells is suitability for infusion.

24. A device according to claim 1, wherein the biological composition comprises platelets.

25. A device according to claim 24, wherein treatment of the biological composition with a device over a period of about 5 days results in less than about a 10% loss in platelet count.

26. A device according to claim 25, wherein the device further comprises a container that is compatible with the biological composition, and wherein the inert matrix is a synthetic polymer fiber comprising a polymer core with a high melting point surrounded by a sheath with a lower melting temperature, and wherein the adsorbent particles are polyaromatic adsorbents comprising a hypercrosslinked polystyrene network, and wherein the surface area of the particles is greater than about 750 m²/g, and wherein the adsorbent particles are immobilized in the matrix.

27. A device according to claim 26, wherein the desired biological activity maintained by the cells is suitability for infusion.

28. A device according to claim 27, wherein the biological composition is treated with the device at a temperature of about 22°

C. for between 0.5 hour and 7 days.

29. A device according to claim 26, wherein the low molecular weight compound is an acridine derivative, a psoralen derivative or a dye.

30. A device according to claim 26, wherein the low molecular weight compound is a biological response modifier.

31. A device according to claim 30, wherein the biological response modifier is activated complement.

32. A device according to claim 1, wherein the biological composition comprises red blood cells.

33. A device according to claim 32, wherein treatment of the biological composition with the device over a period of up to 35 days results in less than about 1% hemolysis.

34. A device according to claim 33, wherein the device further comprises a container that is compatible with the biological composition, and wherein the inert matrix is a synthetic polymer fiber comprising a polymer core with a high melting point surrounded by a sheath with a lower melting temperature, and wherein the adsorbent particles are activated carbon particles derived from a synthetic source, and wherein the diameter of the particles ranges from about 300 μ

m to about 900 μ

m, and wherein the surface area of the particles is greater than about 750 m^2/g, and wherein the adsorbent particles are immobilized in the matrix.

35. A device according to claim 34, wherein the desired biological activity maintained by the cells is suitability for infusion.

36. A device according to claim 34, wherein the low molecular weight compound is an acridine derivative, a psoralen derivative or a dye.

37. A device according to claim 34, wherein the low molecular weight compound is a biological response modifier.

38. A device according to claim 37, wherein the biological response modifier is activated complement.

39. A method for reducing the concentration of a low molecular weight compound in a biological composition containing cells comprising treating the biological composition with a device of claim 1 or 34 for between 0.5 hour and 5 weeks, wherein the cells in the biological composition treated with the device substantially maintain their desired biological activity.

40. A method according to claim 39, wherein the cells in the biological composition treated with the device substantially maintain their desired biological activity after being treated with the device for between 1.0 hour and 5 weeks.

41. A method according to claim 39, wherein the low molecular weight compound is an acridine derivative.

42. A method according to claim 39, wherein the low molecular weight compound is a psoralen derivative.

43. A method according to claim 39, wherein the low molecular weight compound is a dye.

44. A method according to claim 39, wherein the low molecular weight compound is a biological response modifier.

45. A method according to claim 44, wherein the biological response modifier is activated complement.

46. A method according to claim 39, wherein the biological composition is first treated with the device at a temperature of about 22°

C. for between about 0.5 and about 36 hours.

47. A method according to claim 39, wherein the biological composition is first treated with the device at a temperature of about 22°

C. for between about 0.5 and about 24 hours.

48. A method according to claim 39, wherein the biological composition is first treated with the device at a temperature of about 22°

C. for between about 0.5 and about 12 hours.

49. A method according to claim 47, wherein the blood product i s subsequently treated with the device at a temperature of about 4°

C. for up to 5 weeks.

50. A biological composition containing cells, wherein the biological composition is suitable for infusion, and wherein the biological composition is produced by treating a biological composition containing cells with a device according to claim 1 or 34 for a period between about 0.5 hour and 5 weeks.

52. A method for reducing the concentration of a low molecular weight compound in a biological composition containing cells comprising treating the biological composition with a device according to claim 26 for between about 0. 5 hours and about 7 days, wherein the cells in the biological composition treated with the device substantially maintain their desired biological activity.

51. A device for reducing the concentration of small organic compounds in a blood product while substantially maintaining a desired biological activity of the blood product, the device comprising highly porous adsorbent particles, wherein the adsorbent particles are immobilized by an inert matrix.