Controlling microdrop dispensing apparatus

• US 20040185569A1
• Filed: 12/23/2003
• Published: 09/23/2004
• Est. Priority Date: 02/02/2001
• Status: Abandoned Application

• Current Assignee: PerkinElmer LAS, Inc. (Perkinelmer Incorporated)
• Original Assignee: PerkinElmer LAS, Inc. (Perkinelmer Incorporated)
• Inventors: Hahn, Christopher M., Zweifel, Ronald A.
• Application Number:
  US10/746,039
• Publication Number:
  US 20040185569A1
• Time in Patent Office: Days
• US Class Current: 436/43
• CPC Class Codes: B01L 3/0262 using touch-off at substrate or container
• CPC Class Codes: B01L 3/0268 using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
• CPC Class Codes: G01N 2035/1039 Micropipettes, e.g. microcapillary tubes
• CPC Class Codes: G01N 35/1011 Control of the position or alignment of the transfer device
• CPC Class Codes: G01N 35/1016 Control of the volume dispensed or introduced
• CPC Class Codes: Y10T 436/11 Automated chemical analysis

Abstract

A method and apparatus for preventing or limiting damage to capillaries used to dispense microdrops measures the voltage produced by a piezoelectric transducer when the capillary contacts a solid surface or the phase shift occurring when the piezoelectric transducer is operated at its resonant frequency. After distinguishing the voltage created from such contact from the voltage produced from unrelated random sources, corrective action is taken, in one aspect by stopping the relative movement of the capillary and the surface being contacted. The method and apparatus may also be employed to determine the position of a solid or liquid surface. In one embodiment, the method and apparatus of the invention is used to detect contact of the capillary with very small liquid droplets.

Claims

1. In a capillary for dispensing microdrops of liquid by applying pressure to said liquid with a piezoelectric transducer disposed on said capillary and actuated by a voltage pulse, the improvement comprising means for measuring the voltage change produced by said transducer resulting from contact of said capillary with a surface.

2. A capillary of claim 1, wherein said produced voltage is distinguished from voltage produced by said transducer from random sources unrelated to dispensing liquids or contacting of said capillary with surfaces.

3. A capillary of claim 1 wherein said voltage produced by said piezoelectric transducer is used to prevent further contact of said capillary with a surface by stopping movement of said capillary relative to said surface.

8. A capillary of claim 1 wherein said surface is a liquid surface.

9. A capillary of claim 8 wherein said liquid surface is a liquid droplet.

4. In a capillary for dispensing microdrops of liquid by applying pressure to said liquid with a piezoelectric transducer disposed on a capillary and actuated by a voltage pulse, the improvement comprising means for supplying an oscillating voltage to said capillary at its resonant frequency and establishing a reference signal corresponding to the resonant frequency of said capillary and for measuring the phase shift between said capillary and said reference frequencies when said capillary contacts a surface.

5. A capillary of claim 4 wherein said phase shift resulting from contact of said capillary with a surface is used to stop movement of said capillary relative to said surface.

6. A capillary of claim 4 wherein said surface is a liquid surface.

7. A capillary of claim 6 wherein said liquid surface is a liquid droplet.

10. A method of detecting contact of a liquid surface by a capillary for dispensing liquid by action of a piezoelectric transducer disposed on said capillary comprising:

(a) providing an AC voltage to said capillary at a frequency corresponding to the resonant frequency of the capillary;

(b) providing the AC voltage of (a) as a reference frequency, whereby the frequencies of the capillary and the reference are in phase;

(c) detecting contact of a liquid surface by said capillary by determining the phase shift between the reference frequency of (b) and the new resonant frequency of said capillary resulting from contact of said capillary with a liquid surface; and

thereafter

(d) adjusting the frequency of the AC voltage of (a) until it matches the new resonant frequency of the capillary resulting from the contact with the liquid surface.

11. A method of claim 10 where in the frequency of step (a) corresponding to the capillary and the reference frequency of step (b) are separately amplified.

12. A method of claim 11 wherein said amplifiers supply amplified analog signals to logic chip converters that convert the analog signals to square waves.

13. A method of claim 12 wherein said logic chip converters supply square waves to an exclusive OR gate where the phase relationship of the square waves are compared.

14. A method of claim 13 wherein the exclusive OR gate supplies a pulsed signal to a binary counter when the square waves are not in phase.

15. A method of claim 14 wherein the output of said binary counter is converted to an analog DC voltage.

16. A method of claim 15 wherein said analog DC voltage is supplied to a voltage controlled oscillator (VCO) that produces the AC voltage of step (a).

17. A method of claim 10 wherein said liquid surface is a liquid droplet.

18. A circuit for detecting the contact of a liquid surface by a capillary for dispensing liquid by action of a piezoelectric transducer disposed on said capillary comprising:

(a) a voltage controlled oscillator (VCO) for providing an AC voltage at the resonant frequency of said capillary;

(b) a reference amplifier for receiving and amplifying the AC voltage produced by said VCO;

(c) a signal amplifier for receiving the AC voltage produced by said VCO, said AC voltage being reduced by a resistor, said signal amplifier being connected to said capillary;

(d) logic chip converters for receiving the amplified output of said amplifiers of (b) and (c), said logic chip converters converting sine waves from said amplifiers to square waves;

(e) an exclusive OR gate for receiving and comparing the phase relationship of the square waves from the logic chip converters of (d);

said OR gate producing no signal when the square waves are in phase;

(f) a binary counter for receiving the output of said OR gate and producing a pulsed output related to the output received from said OR gate;

(g) an digital-to-analog converter for converting the pulsed output of said binary counter into a DC voltage controlling said VCO.

19. A circuit of claim 18 wherein said liquid surface is a liquid droplet.