Composition for administration of a biologically active compound
First Claim
1. A method for preparing a positively charged compound-containing matrix useful for delivery of a positively charged compound to a mammalian subject, comprising:
- (a) providing a first crosslinkable component having m nucleophilic groups, wherein m≧
2;
(b) providing a second crosslinkable component having n electrophilic groups capable of reaction with the m nucleophilic groups to form covalent bonds, wherein n≧
2 and m+n≧
5;
(c) admixing the first and second crosslinkable components in a basic, aqueous medium to initiate crosslinking and form a crosslinked matrix with an excess of negatively charged electrophilic groups; and
(d) contacting the negatively charged crosslinked matrix with the positively charged compound to allow ionic binding therebetween, wherein the first and second crosslinkable components are biocompatible, synthetic, and nonimmunogenic.
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Accused Products
Abstract
Provided are crosslinked polymer compositions that include a first synthetic polymer containing multiple nucleophilic groups covalently bound to a second synthetic polymer containing multiple electrophilic groups. The first synthetic polymer is preferably a synthetic polypeptide or a polyethylene glycol that has been modified to contain multiple nucleophilic groups, such as primary amino (—NH2) or thiol (—SH) groups. The second synthetic polymer may be a hydrophilic or hydrophobic synthetic polymer, which contains or has been derivatized to contain, two or more electrophilic groups, such as succinimidyl groups. The compositions may further include other components, such as naturally occurring polysaccharides or proteins (such as glycosaminoglycans or collagen) and/or biologically active agents. Also disclosed are methods for using the crosslinked polymer compositions to effect adhesion between a first surface and a second surface; to effect tissue augmentation; to prevent the formation of surgical adhesions; and to coat a surface of a synthetic implant.
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Citations
33 Claims
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1. A method for preparing a positively charged compound-containing matrix useful for delivery of a positively charged compound to a mammalian subject, comprising:
-
(a) providing a first crosslinkable component having m nucleophilic groups, wherein m≧
2;
(b) providing a second crosslinkable component having n electrophilic groups capable of reaction with the m nucleophilic groups to form covalent bonds, wherein n≧
2 and m+n≧
5;
(c) admixing the first and second crosslinkable components in a basic, aqueous medium to initiate crosslinking and form a crosslinked matrix with an excess of negatively charged electrophilic groups; and
(d) contacting the negatively charged crosslinked matrix with the positively charged compound to allow ionic binding therebetween, wherein the first and second crosslinkable components are biocompatible, synthetic, and nonimmunogenic. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33)
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Specification