Afinity biosensor for monitoring biological process
First Claim
1. An optical fiber surface plasmon resonance (SPR) biosensor for observation of a biological marker in a biological fluid wherein said biological marker is a first member of a binding pair, and said biosensor comprises:
- a. an SPR probe surface having immobilized thereon a second member of said binding pair;
b. a spectrophotometric means for receiving a first signal from said probe surface and a second signal from said implanted probe surface, said second signal being received at a time after binding of said first and second members of said binding pair on said probe surface; and
c. means for comparing properties of said first received signal and said second received signal to determine the presence of said biological marker.
2 Assignments
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Accused Products
Abstract
An optical biosensor carries one or more affinity legends or binding members that bind specifically to a marker being monitored. Light directed along optic fibers illuminates a surface plasmon resonance (SPRN) probe surface on which is immobilized the binding member. A spectrophotometer receives light reflected back along the fiber optic path and provides wavelength information indicative of the absence or presence of surface plasmon resonance indicative of the bound marker in known SPR manner. The probe is used in vitro or in vivo. When used in vivo the fiberoptic light path comprises a catheter that directs the probe to an implant site. For in vivo implantation a housing houses the probe at the implant site and is adapted to filter out larger particles that would adversely affect with the spectral analysis. In one embodiment the probe has two regions on its surface. The first region has no immobilized binding member. The second region does have the binding member immobilized on it. Light returned from the first and second regions can be compared. The presence or absence of a marker bound by the binding member on the second surface is apparent in the similarity or dissimilarity of the spectral information returned from the two regions. The probe can monitor blood, spinal fluid, mucus membrane, wound tissue, implanted organs, urine and other substances for the presence of a marker which may be indicative of a medical condition in an animal or human subject.
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Citations
37 Claims
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1. An optical fiber surface plasmon resonance (SPR) biosensor for observation of a biological marker in a biological fluid wherein said biological marker is a first member of a binding pair, and said biosensor comprises:
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a. an SPR probe surface having immobilized thereon a second member of said binding pair;
b. a spectrophotometric means for receiving a first signal from said probe surface and a second signal from said implanted probe surface, said second signal being received at a time after binding of said first and second members of said binding pair on said probe surface; and
c. means for comparing properties of said first received signal and said second received signal to determine the presence of said biological marker. - View Dependent Claims (2, 3, 4, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
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- 5. A biocompatible screen for housing an implanted medial device in an individual, comprising an elastomeric screen formed by a lithographic process, said screen having penetrating holes of between 1 and 50 microns in diameter.
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35. A method of monitoring a human or animal subject for the presence or absence of a marker indicative of the presence or absence of a medical condition comprising:
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a. providing an SPR probe having a first surface with a binding member adherent thereto, the binding member being effective to bind to the marker;
b. providing an optical path to the first surface;
c. locating the probe at a location of interest in or on the subject;
d. directing light to the first surface along the optical path;
e. observing the light retrieved from the probe for spectral indications of the presence or absence of the marker. - View Dependent Claims (36, 37)
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Specification