Immersed microarrays in conical wells

• US 20040191810A1
• Filed: 12/16/2003
• Published: 09/30/2004
• Est. Priority Date: 12/17/2002
• Status: Abandoned Application

• Current Assignee: Affymetrix, Inc. (Thermo Fisher Scientific Incorporated)
• Original Assignee: Affymetrix, Inc. (Thermo Fisher Scientific Incorporated)
• Inventors: Yamamoto, Melvin
• Application Number:
  US10/738,535
• Publication Number:
  US 20040191810A1
• Time in Patent Office: Days
• US Class Current: 435/6.11
• CPC Class Codes: B01J 2219/00315 Microtiter plates
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00475 Sheets
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00662 Two-dimensional arrays within two-dimensional arrays
• CPC Class Codes: B01J 2219/00668 Two-dimensional arrays within three-dimensional arrays
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01L 2200/026 Fluid interfacing between devices or objects, e.g. connectors, inlet details
• CPC Class Codes: B01L 2200/0642 Filling fluids into wells by specific techniques
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 60/14 for creating libraries

Abstract

In an embodiment of the invention a microarray plate comprises a body with a plurality of conical wells, each well having a microarray, wherein the microarrays are captured at the bottom of each well with the use of a frame. A window is connected to the frame to attach the frame with the microarrays.

Claims

1. A microarray plate comprising:

a body comprising a plurality of conical wells;

at least one microarray;

a frame with multiple openings;

wherein the frame is connected to the body to capture the at least one microarray at the bottom of the conical wells;

a optically clear window connected to frame at the bottom of the body.

2. The microarray plate of claim 1 wherein the body has fluidic openings.

3. The microarray plate of claim 2 wherein the body has two fluidic openings leading to the microarray.

4. The microarray plate of claim 3 wherein the fluidic openings are of different sizes.

5. The microarray plate of claim 3 wherein the fluidic openings are located on diagonal corners of the at least one microarray.

6. The microarray plate of claim 3 wherein the frame has perforations that interact with the fluidic openings.

7. The microarray plate of claim 1 wherein the at least one microarray has an active probe surface.

8. The microarray plate of claim 7 wherein the at least one microarray can be captured with its active probe surface up.

9. The microarray plate of claim 7 wherein the at least one microarray can be captured with its active probe surface down.

10. The microarray plate of claim 1 wherein the at least one microarray are gene expression arrays.

11. The microarray plate of claim 1 wherein the at least one microarray are SNP genotyping arrays.

12. A microarray plate comprising:

a body comprising a plurality of conical wells;

at least one microarray;

wherein the at least microarray is captured at the bottom of the well; and

an optically clear window connected to the bottom of the well.

13. The microarray plate of claim 12 wherein the window is molded to the body.

14. The microarray plate of claim 12 wherein the window is fixed to the body with an adhesive.

15. The microarray plate of claim 12 wherein the microarray has an active probe surface.

16. The microarray plate of claim 15 wherein the at least one microarray is captured at the bottom of the wells with its active surface up.

17. The microarray plate of claim 15 wherein the at least one microarray is captured at the bottom of the well with its active surface down.

18. The microarray plate of claim 12 wherein the at least one microarray are gene expression arrays.

19. The microarray plate of claim 12 wherein the at least one microarray are SNP genotyping arrays.

20. A method for constructing a microarray plate comprising:

providing a body comprising a plurality of conical wells;

providing at least one microarray;

capturing the at least one microarray to the bottom of the conical wells of the body with the use of a frame with multiple openings; and

attaching an optically clear window to the frame at the bottom of the body.

21. The method of claim 20 wherein the body has fluidic openings.

22. The method of claim 20 wherein the body has two fluidic openings leading to the microarray.

23. The method of claim 22 wherein the fluidic openings are of different sizes.

24. The method of claim 22 wherein the fluidic openings are located on diagonal corners of the at least one microarray.

25. The method of claim 20 wherein the at least one microarray has an active probe surface.

26. The method of claim 25 wherein the capturing can be done with the active probe surface of the at least one microarray up.

27. The method of claim 20 wherein the at least one microarray are gene expression arrays.

28. The method of claim 20 wherein the at least one microarray are SNP genotyping arrays.