Method for producing target substance

• US 20040191875A1
• Filed: 03/04/2004
• Published: 09/30/2004
• Est. Priority Date: 03/04/2003
• Status: Active Grant

• Current Assignee: Ajinomoto Co., Inc.
• Original Assignee: Ajinomoto Co., Inc.
• Inventors: Takeshita, Ryo, Yasueda, Hisashi
• Granted Patent: US 7,160,704 B2
• Time in Patent Office: Days
• US Class Current: 435/106
• CPC Class Codes: C12N 9/0006 acting on CH-OH groups as donors (1.1)
• CPC Class Codes: C12N 9/1051 Hexosyltransferases (2.4.1)
• CPC Class Codes: C12N 9/90 Isomerases (5.)
• CPC Class Codes: C12P 13/08 Lysine; Diaminopimelic acid; Threonine; Valine

Abstract

The present invention discloses a method for producing a target substance using a coryneform bacterium comprising culturing a coryneform bacterium having an ability to produce the target substance in a medium, resulting in accumulation of the target substance in the medium or cells of the bacterium, and collecting the target substance from the medium or the cells of the bacterium. Also disclosed is a coryneform bacterium which is introduced with a methanol dehydrogenase gene and which has enhanced activities of hexulose phosphate synthase and phosphohexuloisomerase, and to which an ability to utilize methanol is imparted or which has enhanced ability to utilize methanol, and the medium contains methanol as a carbon source.

Claims

1. A method for producing a target substance using a coryneform bacterium comprising:

(A) culturing a coryneform bacterium having an ability to produce said target substance in a medium, resulting in accumulation of the target substance in the medium or cells of the bacterium, and

(B) collecting the target substance from the medium or the cells of the bacterium, wherein a methanol dehydrogenase gene, hexulose phosphate synthase gene and phosphohexuloisomerase gene are introduced into said coryneform bacterium, and said bacterium is modified so that an ability to utilize methanol is imparted, and the medium contains methanol as a carbon source.

2. The method according to claim 1, wherein said bacterium is further introduced with a gene encoding a methanol dehydrogenase activity promoting factor.

3. The method according to claim 1, wherein said target substance is an L-amino acid.

4. The method according to claim 3, wherein said L-amino acid is L-lysine.

5. The method according to claim 4, wherein said bacterium belongs to the genus Corynebacterium.

6. The method according to claim 5, wherein said coryneform bacterium is Corynebacterium glutamicum.

7. A coryneform bacterium which is introduced with a methanol dehydrogenase gene, hexulose phosphate synthase gene and phosphohexuloisomerase gene, and wherein said bacterium is modified so that an ability to utilize methanol is imparted.

8. The coryneform bacterium according to claim 7, which is further introduced with a gene encoding a methanol dehydrogenase activity-promoting factor.

9. The coryneform bacterium according to claim 8, which belongs to the genus Corynebacterium.

10. The coryneform bacterium according to claim 9, which is Corynebacterium glutamicum.