Polyvalent imprinted polymer and process of preparation thereof

• US 20040192869A1
• Filed: 03/31/2003
• Published: 09/30/2004
• Est. Priority Date: 03/31/2003
• Status: Active Grant

• Current Assignee: Council of Scientific & Industrial Research
• Original Assignee: Council of Scientific & Industrial Research
• Inventors: Kulkarni, Mohan Gopalkrishna, Khandare, Jayant Jagannath
• Granted Patent: US 7,041,762 B2
• Time in Patent Office: Days
• US Class Current: 526/264
• CPC Class Codes: B01J 20/26 Synthetic macromolecular compounds
• CPC Class Codes: B01J 20/261 obtained by reactions only involving carbon to carbon unsaturated bonds macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds per se C08F
• CPC Class Codes: B01J 20/268 Polymers created by use of a template, e.g. molecularly imprinted polymers
• CPC Class Codes: B01J 20/3057 Use of a templating or imprinting material molecularly imprinted polymers B01J20/268; filling pores of a substrate or matrix followed by the removal of the substrate or matrix
• CPC Class Codes: C08F 20/58 containing oxygen in addition to the carbonamido oxygen , e.g. N-methylolacrylamide, N-acryloylmorpholine

Abstract

The present invention relates to a polyvalent imprinted polymer useful in medicine and biotechnology and to a process for the preparation thereof More particularly it relates to the said polymer of formula (1) herein below

wherein R is H, CH₃, C₂H₅, C₆H₅, R₁is H, CH₃, C₂H₅, C₆H₅; R₂ is H, CH₃, C₂H₅, C₆H₅X is based on 4-Amino Butyric Acid (4-ABa), 6-Amino Caproic Acid (6-ACA), 8-Amino Octanoic Acid (8-AOA), 10-Amino Decanoic Acid (10-ADA), 11-Amino Undecanoic Acid (11-ADA), n is from 3 to 50, L is OH, NH₂, OCH₃, NH—CH—(CH₃)₂, CI, OCH₂, CH═CH₂, O—CH═CH₂; and Y is N-Acetyl Glucosamine (NAG), mannose, galactose, sialic acid, fructose, ribulose, erythrolose, xylulose, psicose, sorbose tagaose, glucopyranose, fructofuranose, deoxyribose, galactosamine, sucrose, lactose, isomaltose, maltose, cellobiose, cellulose and amylose; Z is based on chain terminating agent such as Mercapto Ethanol, Mercapto, Mercapto Propanol, Mercaptoethylamine HCl,

Claims

1. An imprinted polyvalent polymer of formula (1)

2. A process for preparation of the polyvalent imprinted polymer of the formula 1

3. A process as claimed in claim 2 wherein the biomolecule is selected from the group consisting of enzymes, proteins, bacteria and viruses.

4. A process as claimed in claim 3 wherein the bacteria is selected from the group consisting of E. coli, B. subtilis, S.aurius, micobacterium, sreptococci, pnemonococci.

5. A process as claimed in claim 3 wherein the virus is selected from the group consisting of rotavirus, shiga, influenza, mumps and HIV.

6. A process as claimed in claim 3 wherein the enzyme is selected from the group consisting of lysozyme, trypsin, chymotrypsin, lipase, esterase, amylase, ovalbumin and bovine serum albumin.

7. A process as claimed in claim 2 wherein the biomolecule is a lectin comprising wheat germ agglutinin (WGA).

8. A process as claimed in claim 2 wherein the solvent is selected from water, Dimethyl formamide, Tetrahydrofuran, isobutanol, methanol, ethanol, ethyl acetate, 1,4 dioxane, and benzene.

9. A process as claimed in claim 2 wherein the ratio of biomolecule to the affinity monomer is in the range of 10:

1 to 1.10.

10. A process as claimed in claim 2 wherein the ratio of the biomolecule to the affinity monomer is 1:

1 (w/w).

11. A process as claimed in claim 2 wherein the comonomer of formula 3 is selected from the group consisting of acrylic acid, methacrylic acid, methacryloyl chloride, acrylamide, N-isopropyl acrylamide (NIP A), 2-acrylamida-2 methyl propanesulphonic acid (AMPS), acryloyl chloride, acryloyl moxpholin, vinyl pyrrolidone, styrene, allyl alcohol and allyl amine.

12. A process as claimed in claim 2 wherein the amount of comonomer of formula 3 in the polymerization mixture is in the range of 5 to 80% by weight of the total feed.

13. A process as claimed in claim 12 wherein the amount of comonomer of formula 3 in the polymerization mixture is in the range of 45 to 55% by weight of the total feed.

14. A process as claimed in claim 2 wherein the polymerization initiator is selected from the group consisting of ammonium persulfate, ceric ammonium sulfate, potassium persulfate, and azobis isobutyro nitrile.

15. A process as claimed in claim 2 wherein the amount of polymerization initiator is in the range of 0.1% to 1% (w/w) of total feed.

16. A process as claimed in claim 2 wherein the accelerator is selected from ethylene diamine and N,N,N,N-tetramethylethylenediamine.

17. A process as claimed in claim 2 wherein the amount of polymerization accelerator is in the range of 1% to 4% by (w/w) of total feed.

18. A process as claimed in claim 2 wherein the non-solvent used for precipitation of polyvalent imprinted polymer is selected from the group consisting of diethyl ether, acetone, hexane and hot water.

19. A process as claimed in claim 2 wherein the heating of the solution is carried out at a temperature in the range of 20°

C. to 60°

C.

20. A process as claimed in claim 2 wherein the biomolecule is a lysozyme having a molecular weight from 14,000 to 15,000.

21. A process as claimed in claim 2 wherein the affinity monomer is selected from the group consisting of N-Acetyl Glucosamine, sialic acid, galactose, mannose, galactose, sialic acid, fructose, ribulose, erythrolose, xylulose, psicose, sorbose, tagatose, glucopyranose, fructofuranose, deoxyribose, galactosamine, sucrose, lactose, isomnaltose, maltose, cellobiose, cellulose and amylose.

22. A process as claimed in claim 2 wherein the polyvalent imprinted polymer containing ligand is synthesized by free radical polymerization.