Process for detecting the presence or quantity of enzymatic activity in a sample
3 Assignments
0 Petitions
Accused Products
Abstract
This invention provides for labeling reagents, labeled targets and processes for preparing labeling reagents. The labeling reagents can take the form of cyanine dyes, xanthene dyes, porphyrin dyes, coumarin dyes or composite dyes. These labeling reagents are useful for labeling probes or targets, including nucleic acids and proteins. These reagents can be usefully applied to protein and nucleic acid probe based assays. They are also applicable to real-time detection processes.
60 Citations
321 Claims
-
1-286. -286. (Canceled)
-
287. A dye composition of the formula
R-Fluorescent Dye wherein R is covalently linked to said Fluorescent Dye comprises two or more members in combination from a) unsaturated aliphatic groups; -
b) unsaturated heterocyclic groups;
c) aromatic groups; and
wherein R is capable of providing a conjugated system or an electron delocalized system with said fluorescent dye. - View Dependent Claims (288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306)
-
-
307. A labeled target having the structure
[R-Dye]— - target
wherein said Dye is a fluorescent dye, wherein R is covalently linked to said Dye, and wherein R comprises two or more members in combination from a) unsaturated aliphatic groups;
b) unsaturated heterocyclic groups;
c) aromatic groups;
and wherein R is capable of providing a conjugated system or an electron delocalized system with said Dye. comprises two or more unsaturated aliphatic groups, unsaturated heterocyclic groups, aromatic groups, or combinations of the foregoing groups and wherein R is covalently attached to said fluorescent dye and is capable of providing a conjugated system or an electron delocalized system with said fluorescent dye, and wherein said target is covalently attached to said Dye or said R. - View Dependent Claims (308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321)
an unsaturated aliphatic group (a) and an aromatic group (c);
oran unsaturated heterocyclic group (b) and an aromatic group (c).
- target
-
316. The labeled target of claim 307, wherein said fluorescent dye comprises an anthracene, a xanthene, a cyanine, a porphyrin, a coumarin or a composite dye.
-
317. The labeled target of claim 307, further comprising a charged or polar R′
- group.
-
318. The labeled target of claim 307, wherein said charged or polar R′
- group increases aqueous solubility of said composition.
-
319. The labeled target of claim 317, further comprising a linker arm attaching said target to said fluorescent dye, said R group or said R′
- group.
-
320. The labeled target of claim 307, wherein said target comprises a protein, a peptide, a nucleic acid, a nucleotide or a nucleotide analog, a receptor, a natural or synthetic drug, a synthetic oligomer, a synthetic polymer, a hormone, a lymphokine, a cytokine, a toxin, a ligand, an antigen, a hapten, an antibody, a carbohydrate, a sugar or an oligo- or polysaccharide.
-
321. The labeled target of claim 320, wherein said ligand comprises biotin, iminobiotin, digoxygenin or fluorescein, and the dye comprises a fluorescent dye.
Specification