Device for referencing fluorescence signals
First Claim
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1. A device for referencing fluorescence signals, comprising:
- an essentially non-fluorescent carrier; and
a plurality of polymer layers applied to the carrier, at least one polymer layer applied to defined regions of the carrier, wherein the at least one polymer layer fluoresces in response to corresponding irradiation, and wherein at least two of the polymer layers differ with regard to their thickness and/or composition.
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Abstract
The invention relates to a device for referencing fluorescence signals and/or calibration of fluorescence detection systems, whereby the device comprises an essentially non-fluorescing support, on which are applied polymer layers in several defined regions and with partly varying thicknesses and/or compositions. Said polymer layers are applied to the support such as to fluoresce after corresponding irradiation and the device may thus be used as a fluorescence standard. The invention further relates to a method for the production of said fluorescence standards.
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Citations
78 Claims
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1. A device for referencing fluorescence signals, comprising:
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an essentially non-fluorescent carrier; and
a plurality of polymer layers applied to the carrier, at least one polymer layer applied to defined regions of the carrier, wherein the at least one polymer layer fluoresces in response to corresponding irradiation, and wherein at least two of the polymer layers differ with regard to their thickness and/or composition. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 73, 74, 75, 76, 77)
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27. A method of manufacturing a device for referencing fluorescence signals, comprising:
applying at least one polymer layer to at least one defined region of an essentially non-fluorescent carrier using a microtechnical process, wherein the at least one polymer layer fluoresces in response to corresponding irradiation. - View Dependent Claims (28, 29, 30, 31, 32, 33)
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34. A method of using a device for online calibration of fluorescence detection systems with regard to at least one of their spatial or temporal resolution, their geometric and/or dynamic characteristics, and their characteristics caused by aging, wherein the device comprises:
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an essentially non-fluorescent carrier; and
a plurality of polymer layers applied to the carrier, at least one polymer layer applied to defined regions of the carrier, wherein the at least one polymer layer fluoresces in response to corresponding irradiation, and wherein at least two of the polymer layers differ with regard to their thickness and/or composition. - View Dependent Claims (35, 36)
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37. A method of using a device for referencing fluorescence signals and/or for cross-system and cross-device and/or cross-test comparison of fluorescence signals, wherein the fluorescent signals are preferably fluorescence signals of interaction studies on the basis of probe arrays, and wherein the device comprises:
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an essentially non-fluorescent carrier; and
a plurality of polymer layers applied to the carrier, at least one polymer layer applied to defined regions of the carrier, wherein the at least one polymer layer fluoresces in response to corresponding irradiation, and wherein at least two of the polymer layers differ with regard to their thickness and/or composition. - View Dependent Claims (38, 39, 40)
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41. A method of using a device for cross-system and cross-device and/or cross-test comparison of cellular localization experiments and tissue slices which are evaluable by fluorescence microscopy, wherein the device comprises:
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an essentially non-fluorescent carrier; and
a plurality of polymer layers applied to the carrier, at least one polymer layer applied to defined regions of the carrier, wherein the at least one polymer layer fluoresces in response to corresponding irradiation, and wherein at least two of the polymer layers differ with regard to their thickness and/or composition.
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42. A fluorescence calibration device, comprising:
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an essentially non-fluorescent carrier having one or more defined regions;
at least one polymer layer applied to the one or more defined regions, wherein the polymer layer is uniform with regard to layer thickness and composition, such that the one or more defined regions fluoresce after corresponding irradiation.
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43-72. -72. canceled
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78-99. -99. canceled
Specification