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Methods and apparatus for biomolecule detection, identification, quantification and/or sequencing

  • US 20040197793A1
  • Filed: 07/24/2003
  • Published: 10/07/2004
  • Est. Priority Date: 08/30/2002
  • Status: Abandoned Application
First Claim
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1. A method comprising:

  • a) obtaining at least one sample suspected of containing one or more target biomolecules;

    b) using the target biomolecule to generate pyrophosphate (PPi) by an enzyme-catalyzed reaction;

    c) using the PPi to produce light by a luciferase dependent process;

    d) accumulating the number of photons produced over a time interval; and

    e) detecting the target biomolecules in the sample.

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