×

Method, primer and kit for determining base type

  • US 20040197803A1
  • Filed: 12/05/2003
  • Published: 10/07/2004
  • Est. Priority Date: 12/06/2002
  • Status: Abandoned Application
First Claim
Patent Images

1. A base type determination method for determining a base type of a monobasic substituted region of a target nucleic acid, the method comprising the steps of:

  • (a) preparing a solution containing a target double-stranded nucleic acid having the monobasic substituted region, a base type determination primer, a DNA polymerase, and dNTPs;

    (b) causing the base type determination primer to hybridize to the target double-stranded nucleic acid in the solution, and causing a primer extension reaction to start progressing from the base type determination primer; and

    (c) analyzing the degree of progress of the primer extension reaction to determine the base type of the substituted region, wherein the base type determination primer consists of a first single-stranded nucleic acid which is capable of, when hybridizing to the target double-stranded nucleic acid, hybridizing to one of two strands of the target double-stranded nucleic acid such that a 3′

    terminal of the primer corresponds to the substituted region of said one strand of the target double-stranded nucleic acid, and wherein the first single-stranded nucleic acid consists of;

    a substitution corresponding region which is located at the 3′

    terminal and consists of one base complementary to any one of predictable types of bases in the substituted region of said one strand of the target double-stranded nucleic acid;

    an uncomplementary region which is located adjacent to the substitution corresponding region on a 5′

    terminal side thereof and consists of two bases uncomplementary to said one strand of the target double-stranded nucleic acid; and

    a complementary region which is located adjacent to the uncomplementary region on the 5′

    terminal side thereof and consists of five or more bases complementary to said one strand of the target double-stranded nucleic acid.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×