Methods and reagents for multiplexed analyses
First Claim
1. A method for detecting one or more target proteins in a sample suspected of containing a plurality of said target proteins, said method comprising:
- (a) incubating an assay medium comprising said sample with a surface of a substrate comprising a plurality of capture agents bound thereto in a predetermined pattern, wherein for each target protein at least two capture agents specific for different binding sites on said target protein are employed and wherein said two capture agents bind to said target protein, said assay medium being incubated under conditions for binding of said capture agents to said target proteins, and (b) examining said substrate surface for the presence of said target proteins.
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Accused Products
Abstract
Methods, devices and kits are disclosed for detecting one or more target proteins in a sample suspected of containing a plurality of the target proteins. An assay medium comprising the sample is incubated with a surface of a substrate comprising a plurality of capture agents bound to the surface in a predetermined arrangement or pattern. For each target protein, at least two capture agents specific for different binding sites on the target protein are employed. The two capture agents are disposed on the surface of the substrate in a spatial relationship so that both bind to a molecule of the target protein, preferably the same molecule of the target protein. The binding to the molecule of the target protein is usually substantially simultaneous. The assay medium is incubated under conditions for binding of the capture agents to the target proteins. The substrate is then examined for the presence of the target proteins.
17 Citations
30 Claims
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1. A method for detecting one or more target proteins in a sample suspected of containing a plurality of said target proteins, said method comprising:
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(a) incubating an assay medium comprising said sample with a surface of a substrate comprising a plurality of capture agents bound thereto in a predetermined pattern, wherein for each target protein at least two capture agents specific for different binding sites on said target protein are employed and wherein said two capture agents bind to said target protein, said assay medium being incubated under conditions for binding of said capture agents to said target proteins, and (b) examining said substrate surface for the presence of said target proteins. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method for determining one or more different target proteins in a sample suspected of containing a plurality of said target proteins, said method comprising:
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(a) incubating an assay medium comprising said sample with a surface of a substrate comprising an array of capture agents bound thereto, wherein at least two capture agents specific for different binding sites on each of said different target proteins are employed and wherein said two capture agents bind to said target protein, said assay medium being incubated under conditions for binding of said capture agents to said target proteins, and (b) examining said substrate surface for the presence of said target proteins. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22)
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23. A device for determining one or more different target proteins in a sample suspected of containing a plurality of said target proteins, said apparatus comprising:
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(a) a solid substrate comprising a surface, and (b) a plurality of capture agents bound to said surface in a predetermined pattern, wherein at least two capture agents specific for different binding sites on each of said target proteins are present and wherein said two capture agents are spatially disposed to both bind to a single molecule of said target protein. - View Dependent Claims (24, 25, 26, 27, 28, 29, 30)
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Specification