Process for preparing novel cyanine dye labeling reagents
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Abstract
This invention provides for labeling reagents, labeled targets and processes for preparing labeling reagents. The labeling reagents can take the form of cyanine dyes, xanthene dyes, porphyrin dyes, coumarin dyes or composite dyes. These labeling reagents are useful for labeling probes or targets, including nucleic acids and proteins. These reagents can be usefully applied to protein and nucleic acid probe based assays. They are also applicable to real-time detection processes.
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Citations
300 Claims
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1-286. -286. (canceled)
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287. A process for preparing a cyanine dye labeling reagent, said process comprising the steps of:
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(a) providing;
(i) a first intermediate compound comprising;
wherein X1 comprises carbon, oxygen, nitrogen or sulfur; and
(ii) a second intermediate compound comprising;
wherein X1 comprises carbon, oxygen, nitrogen or sulfur;
wherein at least one of R1 through R10 comprises a reactive group capable of forming a carbon-carbon linkage with a target, and(ii) linking reagents suitable for linking said first intermediate compound and said second intermediate compound;
(b) forming a reaction mixture comprising said first intermediate compound (i), said second intermediate compound (ii), and said linking reagents under conditions to link (i) and (ii) to form a compound;
wherein at least one of R1 through R10 comprises a reactive group capable of forming a carbon-carbon linkage with a target, and wherein n is an integer of 1, 2 or 3, and wherein X1 and X2 independently comprise carbon, oxygen, nitrogen or sulfur. - View Dependent Claims (288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300)
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Specification