Method for parallel synthesis and transfer of molecules to a substrates
First Claim
1. A method for preparing molecule libraries arranged in a defined pattern on a substrate, characterized by preparation of at least one template die comprising a defined arrangement of template molecules, true-to-location biochemical or chemical in situ synthesis of copy molecules on said template molecules located on the surface of said template die and serving as templates, transfer of the synthesized copy molecules to a solid or polymeric substrate by means of contact printing, retaining the spatial arrangement of said copy molecules on the substrate, reproducibility of the synthetic steps and transfer steps.
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Abstract
The invention relates to a method for highly parallel production of complex molecule libraries on the surface of a substrate with the aid of regeneratable matrix dies. According to prior art, the production of molecule libraries on a support is expensive, and requires a lot of time and a large amount of apparatus. By using regeneratable matrix dies it is possible to simplify and accelerate the production of molecule libraries, especially DNA and protein libraries, in a significant manner. The matrix-10 die is made of an elastic material such as polydimethylsiloxane and includes locally-bound matrix molecules on the surface thereof, said molecules being used as a model for the synthesis of copy molecules. The copy molcules are transferred to the target surface by means of a contact printing method. The matrix molecules remain on the die for further synthesis and print cycles which can repeated several times. The inventive method is suitable for copying and reproducing differently produced molecule arrays.
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33 Claims
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1. A method for preparing molecule libraries arranged in a defined pattern on a substrate, characterized by
preparation of at least one template die comprising a defined arrangement of template molecules, true-to-location biochemical or chemical in situ synthesis of copy molecules on said template molecules located on the surface of said template die and serving as templates, transfer of the synthesized copy molecules to a solid or polymeric substrate by means of contact printing, retaining the spatial arrangement of said copy molecules on the substrate, reproducibility of the synthetic steps and transfer steps.
Specification