Complex technologies using enzymatic protein hydrolysate

• US 20040203134A1
• Filed: 02/05/2003
• Published: 10/14/2004
• Est. Priority Date: 02/06/2002
• Status: Abandoned Application

• Current Assignee: Green Earth Industries, LLC
• Original Assignee: Green Earth Industries, LLC
• Inventors: Pyntikov, Alexander V., Salerno, Salvatore P.
• Application Number:
  US10/358,136
• Publication Number:
  US 20040203134A1
• Time in Patent Office: Days
• US Class Current: 435/252.1
• CPC Class Codes: C12N 1/20 Bacteria; Culture media therefor
• CPC Class Codes: C12P 21/06 produced by the hydrolysis of a peptide bond, e.g. hydrolysate products preparing foodstuffs by protein hydrolysis A23J3/00

Abstract

The present invention comprises various uses of enzymatic protein hydrolysate (“EPH,”). The EPH production process involves the profound enzymatic protein hydrolysis of marine animal biomass, whereby cold-water fish wastes are reacted with viscera containing aggressive enzymes that are effective in even mild (slightly alkaline) medium of water. The EPH may be dried to a powder state that is made of approximately 70%-90% free amino acids, 10%-20% highly molecular peptides and 3%-5% vitamins, minerals and oils. The biological value of the EPH stems from the special mix of nutrients and the method by which they are obtained. EPH is particularly suitable as an enhancer for the acceleration of known biological and industrial processes, and most particularly, processes depending upon bacterial or cellular action. The low cost of this highly nutritious product makes it possible to utilize mixtures of amino acids and other nutrients in bio-industrial processes to make them cost-competitive with competing processes.

Claims

1. A method for accelerating the growth of microorganisms employed in industrial processes, comprising:

adding an enzymatic protein hydrolysate (EPH) to said microorganisms during a growth phase of said microorganisms.

2. The method according to claim 1, wherein said EPH comprises a 70-90 percent mixture, by weight, of free amino acids, a 10-20 percent mix, by weight, of bioactive peptides, and a 3-5 percent mixture, by weight, of vitamins and minerals.

3. The method of claim 2, wherein said amino acids consist of:

taurine, aspartine, threonine, serine, glutamic acid, praline, glycine, alanine, valine, methionine, isoleucine, leucine, tyrosine, phenylaline, oxyhedral proline, omithine, lysine, histidine, arginine.

4. The method of claim 3, wherein said minerals consist of:

cadmium, lead, mercury, arsenic, natrium, potassium, calcium, phosphorous, magnesium, iron, zinc, copper, manganese, selenium, cobalt, molybdenum, and chromium.

5. The method according to claim 1, wherein said microorganisms are bacteria.

6. The method according to claim 2, wherein said microorganisms are bacteria.

7. The method according to claim 3, wherein said microorganisms are bacteria.

8. The method according to claim 4, wherein said microorganisms are bacteria.

9. The method according to claim 1, wherein said microorganisms are yeasts.

10. The method according to claim 2, wherein said microorganisms are yeasts.

11. The method according to claim 3, wherein said microorganisms are yeasts.

12. The method according to claim 4, wherein said microorganisms are yeasts.

13. The method according to claim 1, wherein said microorganisms are fungi.

14. The method according to claim 4, wherein said microorganisms are fungi.

15. The method according to claim 4, wherein said EPH is an additive to the final product of said industrial processes, wherein said product is selected from among the group consisting of:

food colorants, food flavorings, vitamins, vegetable-derived pastes, food thickeners, meat products, fish products, fruits, vegetable preserves, pet foods, food spices, vinegar, lactic acid, salicylic acid, amber, apple, enzymes, chemical substances, and biopolymers.

16. The method according to claim 8, wherein said EPH is added as an accelerant to said bacteria that are utilized in the processing of industrial waste, wherein said waste is selected from the group comprising:

plant biomass, animal biomass, industrial waste, sewage, xenobiotics, and crude oil.

17. The method according to claim 8, wherein said EPH is added as an accelerant to said bacteria that are utilized in the processing of metals—

bioextractive metallurgy—

the processing of metals selected from the group including;

uranium, gold, silver, copper, zinc, cobalt, and rare earth metals.

18. The method according to claim 8, wherein said EPH is added as an accelerant to said bacteria utilized in the production of ethyl alcohol, methyl alcohol, prophyl alcohol, methane gas, acetone, butanol, isopropyl, diesel fuel.

19. A method for enhancing the production of coal, comprising:

adding EPH to any mixture of coal digesting microorganisms during their growth phase.

20. The method according to claim 19, wherein said microorganisms are derived from the digestive tract of termites.