Immunosensor

• US 20040203137A1
• Filed: 04/22/2004
• Published: 10/14/2004
• Est. Priority Date: 07/14/2000
• Status: Active Grant

• Current Assignee: Universal Biosensors Pty Ltd. (Universal Biosensors, Inc.)
• Original Assignee: Universal Biosensors Pty Ltd. (Universal Biosensors, Inc.)
• Inventors: Hodges, Alastair, Chatelier, Ron
• Granted Patent: US 8,685,714 B2
• Time in Patent Office: Days
• US Class Current: 435/287.2
• CPC Class Codes: C12Q 1/004 mediator-assisted
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: G01N 27/3271 Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001
• CPC Class Codes: G01N 33/84 involving inorganic compounds or pH

Abstract

This invention describes a quantitative, inexpensive, disposable immunosensor that requires no wash steps and thus generates no liquid waste. Moreover, in preferred embodiments of the sensor no timing steps are required of the user, and the sensor can be readily adapted to antigen-antibody interactions over a wide kinetic range.

Claims

1. A disposable device for use in detecting a target antigen in a fluid sample having a pH, the device comprising a reaction chamber having an internal surface, a proximal end, and a distal end;

an immobilized antibody that binds to the target antigen fixed within the reaction chamber;

a reporter complex present within the reaction chamber, the complex comprising a probe, wherein the reporter complex mixes with the sample in the reaction chamber;

a detection chamber having a wall, an internal surface, a distal end and a proximal end, the detection chamber being initially configures in a first, unvented, state and further being configured to permit modification to a second, vented, state during use;

a sample ingress at the distal end of the reaction chamber; and

a sample passageway between the distal end of the reaction chamber and the proximal end of the detection chamber, the passageway having dimensions selected to block free flow of fluid from the reaction chamber to the detection chamber when the detection chamber is unvented, while permitting free flow of fluid from the reaction chamber to the detection chamber when the detection chamber is vented.

2-63. -63 Cancelled.