Transgenically produced decorin

• US 20040205832A1
• Filed: 05/04/2001
• Published: 10/14/2004
• Est. Priority Date: 05/05/2000
• Status: Abandoned Application

• Current Assignee: GTC Biotherapeutics, Inc., Integra Lifesciences Corporation (Integra LifeSciences Holdings Corporation)
• Original Assignee: GTC Biotherapeutics, Inc.
• Inventors: Meade, Harry M., Pierschbacher, Michael
• Application Number:
  US09/849,657
• Publication Number:
  US 20040205832A1
• Time in Patent Office: Days
• US Class Current: 800/7
• CPC Class Codes: A01K 2217/05 Animals comprising random inserted nucleic acids (transgenic)
• CPC Class Codes: A01K 2227/102 Caprine
• CPC Class Codes: A01K 2227/105 Murine
• CPC Class Codes: A01K 2267/01 Animal expressing industrially exogenous proteins
• CPC Class Codes: A01K 67/0275 Genetically modified vertebrates, e.g. transgenic
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61P 17/02 for treating wounds, ulcers, burns, scars, keloids, or the like
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: C07K 14/4725 Proteoglycans, e.g. aggreccan
• CPC Class Codes: C12N 15/8509 for producing genetically modified animals, e.g. transgenic

Abstract

Transgenically produced decorin and methods of making and using transgenically produced decorin.

Claims

1. A transgenically produced preparation of decorin.

2. The preparation of claim 1, wherein said decorin is human decorin.

3. The preparation of claim 1, wherein said decorin is produced in a transgenic animal.

4. The preparation of claim 1, wherein said decorin is produced in a transgenic mammal.

5. The preparation of claim 1, wherein said decorin is produced in a transgenic dairy animal.

6. The preparation of claim 1, wherein said decorin is produced in a transgenic goat.

7. The preparation of claim 1, wherein the transgenically produced decorin lacks a GAG chain.

8. The preparation of claim 1, wherein the transgenically produced decorin is made in a mammary gland of a transgenic mammal.

9. A transgenically produced preparation of decorin, wherein less than 30% of the decorin molecules in the preparation have a GAG chain.

10. A method of making a preparation of transgenic decorin comprising:

providing a transgenic organism, which includes a transgene which directs the expression of decorin;

allowing the transgene to be expressed; and

recovering a preparation of transgenically produced decorin, from the organism or from a product produced by the organism.

11. The method of claim 10, wherein said decorin is human decorin

12. The method of claim 10, wherein said decorin is produced in a transgenic animal.

13. The method of claim 10, wherein said decorin is produced in a transgenic mammal.

14. The method of claim 10, wherein said decorin is produced in a transgenic dairy animal.

15. The method of claim 10, wherein said decorin is produced in a transgenic goat.

16. The method of claim 12, the transgenically produced decorin lacks a GAG chain.

17. The method of claim 10, wherein the transgenically produced decorin is made in a mammary gland of a transgenic mammal.

18. A method for providing a transgenic preparation which includes heterologous decorin in the milk of a transgenic mammal comprising:

obtaining milk from a transgenic mammal having introduced into its germline a decorin protein-coding sequence operatively linked to a promoter sequence that result in the expression of the protein-coding sequence in mammary gland epithelial cells, thereby secreting the decorin in the milk of the mammal to provide the preparation.

19. A transgenic organism, which expresses a transgenic decorin and from which a transgenic preparation of decorin can be obtained.

20. A pharmaceutical composition comprising a therapeutically effective amount of transgenic decorin or a transgenic preparation of decorin and a pharmaceutically acceptable carrier.

21. A formulation, which includes a transgenically produced human decorin and at least one other nutritional component.

22. A method of providing decorin to a subject in need of decorin comprising administering transgenically produced decorin or a transgenic preparation of decorin to said subject.

23. The method of claim 22, wherein the subject is suffering from cancer.

24. The method of claim 22, wherein the subject is suffering from invasive skin injuries.