Methods and apparatus for calibrating spectral data
First Claim
1. A method of calibrating spectral data obtained from a tissue sample, the method comprising the steps of:
- (a) obtaining calibration data from a plurality of spaced-apart locations on a calibration target;
(b) obtaining a set of spectral data from spaced-apart locations of a tissue sample, wherein at least some of said spaced-apart locations of said tissue sample correspond to said spaced-apart locations on said calibration target; and
(c) calibrating said spectral data obtained from said tissue sample using said calibration data, thereby to produce calibrated data.
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Abstract
The invention provides methods for calibrating spectral data acquisition systems. These calibration methods produce spectral data sufficiently accurate for use in tissue classification algorithms. The invention improves the accuracy of spectral-based tissue classification schemes, in part, by properly accounting for spatial variations, instrument-to-instrument variations, and patient-to-patient variations in the acquisition of spectral data from tissue samples. Effects that are accounted for include, for example, stray light effects, electronic background effects, variation in light energy delivered to a tissue sample, spatial heterogeneities of the illumination source, chromatic aberrations of the scanning optics, variation in wavelength response of the collection optics, and efficiency of the collection optics.
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Citations
32 Claims
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1. A method of calibrating spectral data obtained from a tissue sample, the method comprising the steps of:
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(a) obtaining calibration data from a plurality of spaced-apart locations on a calibration target;
(b) obtaining a set of spectral data from spaced-apart locations of a tissue sample, wherein at least some of said spaced-apart locations of said tissue sample correspond to said spaced-apart locations on said calibration target; and
(c) calibrating said spectral data obtained from said tissue sample using said calibration data, thereby to produce calibrated data. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method of calibrating spectral data obtained from a tissue sample, the method comprising the steps of:
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(a) obtaining a first set of calibration data from a plurality of spaced-apart locations on a first calibration target;
(b) obtaining a second set of calibration data from a plurality of spaced-apart locations on a second calibration target;
(c) obtaining a set of spectral data from spaced-apart locations of a tissue sample, wherein at least some of said spaced-apart locations of said tissue sample correspond to said spaced-apart locations on said first calibration target and said spaced-apart locations on said second calibration target; and
(c) calibrating said spectral data obtained from said tissue sample using said first set of calibration data and said second set of calibration data, thereby to produce calibrated data. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22)
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23. A method of calibrating spectral data obtained from a tissue sample, the method comprising the steps of:
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(a) obtaining calibration data from a plurality of spaced-apart locations on a calibration target using an optical instrument with a first attached disposable component;
(b) obtaining a set of spectral data from spaced-apart locations of a tissue sample, wherein at least some of said spaced-apart locations of said tissue sample correspond to said spaced-apart locations on said calibration target; and
(c) calibrating said spectral data obtained from said tissue sample using said calibration data, thereby to produce .calibrated data.;
- View Dependent Claims (24, 25, 26, 27)
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28. A method of correcting spectral data from a tissue sample for stray light internal to an optical instrument, the method comprising the steps of:
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(a) obtaining a first set of spectral data using a target and using a light source internal to an optical instrument, wherein said instrument yields a residual optical signal;
(b) obtaining a second set of spectral data using a light source internal to said optical instrument, with no external light source;
(c) obtaining a third set of spectral data from a tissue sample; and
(d) adjusting said third set of data using a subset of said first set of data and a subset of said second set of data. - View Dependent Claims (29, 30, 31, 32)
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Specification