Compounds and methods for analyzing the proteome
First Claim
Patent Images
1. A gradient array, comprising:
- a 2-dimensional array of moieties X and optionally Y; and
a surface Z for presenting moieties X and Y, wherein;
the surface Z comprises a solid support;
the X and Y moieties are arranged in a two dimensional array of continuous gradients of one or more properties of X and Y;
the properties of moiety X are in a gradient along the X-axis;
the properties of moiety Y are in a gradient along the Y-axis;
the X moieties are each independently selected to bind to biomolecules covalently or with sufficiently high affinity so that the resulting complexes of biomolecule/capture compounds are stable under conditions of mass spectrometric analysis; and
the Y moieties are each independently selected to increase the selectivity of the binding by moiety X.
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Abstract
Gradient arrays for the analysis of biomolecules are provided. In particular, arrays and methods are provided for analyzing complex protein mixtures, such as the proteome. The arrays provide bifunctional reagents that allow for the separation and isolation of complex protein mixtures. Automated instruments for performing the methods are also provided.
135 Citations
19 Claims
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1. A gradient array, comprising:
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a 2-dimensional array of moieties X and optionally Y; and
a surface Z for presenting moieties X and Y, wherein;
the surface Z comprises a solid support;
the X and Y moieties are arranged in a two dimensional array of continuous gradients of one or more properties of X and Y;
the properties of moiety X are in a gradient along the X-axis;
the properties of moiety Y are in a gradient along the Y-axis;
the X moieties are each independently selected to bind to biomolecules covalently or with sufficiently high affinity so that the resulting complexes of biomolecule/capture compounds are stable under conditions of mass spectrometric analysis; and
the Y moieties are each independently selected to increase the selectivity of the binding by moiety X. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. A collection of capture compounds, comprising a plurality of capture compounds, wherein each capture compound[[,]] comprises a moiety Z for presenting moieties X and Y, wherein the moiety Z comprises a solid support wherein the X and Y moieties are arranged in a two dimensional array of continuous gradients of one or more properties;
- a plurality of X moieties that are selected to bind to biomolecules covalently or with sufficiently high affinity so that the resulting complexes of biomolecule/capture compounds are stable under conditions of mass spectrometric analysis and a plurality of Y moieties that increase the selectivity of the binding by X such that there is selectivity along the Y axis of the two dimentional dimensional array.
- View Dependent Claims (19)
Specification