Method for sequencing nucleic acids by observing the uptake of nucleotides modified with bulky groups
First Claim
1. An apparatus comprising:
- a. an analysis chamber containing one or more structures;
b. one or more reagent reservoirs in fluid communication with the analysis chamber;
c. a detection unit operably coupled to the structures; and
d. a data processing and control unit.
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Accused Products
Abstract
The present methods and apparatus 100 concern nucleic acid 214 sequencing by incorporation of nucleotides 218 into nucleic acid strands 220. The incorporation of nucleotides 218 is detected by changes in the mass and/or surface stress of the structure 116, 212. In some embodiments of the invention, the structure 116, 212 comprises one or more nanoscale or microscale cantilevers. In certain embodiments of the invention, each different type of nucleotide 218 is distinguishably labeled with a bulky group and each incorporated nucleotide 218 is identified by the changes in mass and/or surface stress of the structure 116, 212 upon incorporation of the nucleotide 218. In alternative embodiments of the invention only one type of nucleotide 218 is exposed at a time to the nucleic acids 214, 220. Changes in the properties of the structure 116, 212 may be detected by a variety of methods, such as piezoelectric detection, shifts in resonant frequency of the structure 116, 212, and/or position sensitive photodetection.
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Citations
22 Claims
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1. An apparatus comprising:
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a. an analysis chamber containing one or more structures;
b. one or more reagent reservoirs in fluid communication with the analysis chamber;
c. a detection unit operably coupled to the structures; and
d. a data processing and control unit. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. An apparatus comprising:
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a) an analysis chamber containing at least one cantilever;
b) one or more nucleic acids molecules attached to the at least one cantilever;
c) a detection unit to detect, deflection of the at least one cantilever; and
d.) a data processing and control unit. - View Dependent Claims (9, 10, 11, 12, 13, 14, 15)
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16. An apparatus comprising:
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a) an analysis chamber containing at least one cantilever;
b) one or more nucleic acids molecules attached to the at least one cantilever;
c) a piezoresistive resistor embedded at the fixed end of at least one cantilever;
d) a detection unit to detect deflection of the at least one cantilever; and
e) a data processing and control unit. - View Dependent Claims (17, 18, 22)
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19. An apparatus comprising:
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a) an analysis chamber containing at least one cantilever;
b) the at least one cantilever coated with a substance;
c) one or more nucleic acids molecules associated with the at least one cantilever;
d) one or more polymerases in the analysis chamber;
d) a detection unit to detect deflection of the at least one cantilever; and
e) a data processing and control unit. - View Dependent Claims (20, 21)
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Specification