Thermal cycling system and method of use

• US 20040209331A1
• Filed: 11/21/2003
• Published: 10/21/2004
• Est. Priority Date: 07/16/2001
• Status: Abandoned Application

• Current Assignee: BioFire Diagnostics Incorporated (Compagnie Merieux Alliance SAS)
• Original Assignee: BioFire Diagnostics Incorporated (Compagnie Merieux Alliance SAS)
• Inventors: Ririe, Kirk
• Application Number:
  US10/478,453
• Publication Number:
  US 20040209331A1
• Time in Patent Office: Days
• US Class Current: 435/91.2
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/1822 using Peltier elements
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: B01L 3/505 flexible containers not provided for above
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: B01L 7/525 with physical movement of samples between temperature zones
• CPC Class Codes: B01L 7/5255 by moving sample containers
• CPC Class Codes: C12Q 1/686 Polymerase chain reaction [PCR]

Abstract

A temperature cycling system (10, 110) is provided for repeatedly heating and cooling a reaction mixture (16). The system (10, 110) includes a first heater (27) and a second heater (28) each movable between a first orientation in which the first or second heater (27, 28) affects the temperature of the reaction mixture (16) and a second orientation in which the first or second heater (27, 28) does not substantially affect the temperature of the reaction mixture (16). During temperature cycling, the second heater (28) is in the second orientation when the first heater (27) is in the first orientation, and the second heater (28) is in the first orientation when the first heater (27) is in the second orientation.

Claims