Treatment for diabetes
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Accused Products
Abstract
Methods and compositions for treating diabetes mellitus in a patient in need thereof are provided. The methods include administering to a patient a composition providing a gastrin/CCK receptor ligand, e.g., a gastrin, and/or an epidermal growth factor (EGF) receptor ligand, e.g., TGF-α, in an amount sufficient to effect differentiation of pancreatic islet precursor cells to mature insulin-secreting cells. The composition can be administered systemically or expressed in situ by cells transgenically supplemented with one or both of a gastrin/CCK receptor ligand gene, e.g., a preprogastrin peptide precursor gene and an EGF receptor ligand gene, e.g., a TGF-α gene. The methods also include transplanting into a patient cultured pancreatic islets in which mature insulin-secreting beta cells are proliferated by exposure to a gastrin/CCK receptor ligand and an EGF receptor ligand.
66 Citations
36 Claims
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1-18. -18 (canceled)
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19. A method for treating diabetes mellitus in an individual in need thereof, said method comprising:
administering to said individual a composition providing a gastrin/CCK receptor ligand that stimulates islet cell neogenesis in an amount sufficient to effect differentiation of pancreatic islet precursor cells to mature insulin-secreting cells. - View Dependent Claims (20, 21, 22, 23, 24, 25, 28, 31, 33)
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26. A method for treating diabetes in a patient in need thereof, said method comprising the step of:
ransplanting into said patient pancreatic islets which have been provided ex vivo with a sufficient amount of a gastrin/CCK receptor ligand to induce differentiation of precursor cells in said islets to mature insulin-secreting β
-cells.- View Dependent Claims (27)
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29. A method for enhancing production of mature insulin-secreting cells, said method comprising:
providing to a population of precursor cells an effective amount of a gastrin/CCK receptor ligand to effect differentiation of said precursor cells to mature insulin-secreting cells.
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30. A composition comprising:
pancreatic β
cells, wherein said culture is obtained by providing precursor cells of said pancreatic β
cells with a sufficient amount of a gastrin receptor agonist to induce differentiation of said precursor cells to mature pancreatic β
cells.
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32. A method for stimulating pancreatic islet cell neogenesis in an individual in need thereof, said method comprising:
administering to said individual a composition comprising a gastrin in an amount sufficient to effect differentiation of pancreatic islet precursor cells to mature insulin-secreting islet cells, wherein said composition is administered systemically.
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34. A kit comprising:
one or more containers filled with one or more ingredient for a pharmaceutical composition comprising a gastrin receptor ligand. - View Dependent Claims (35, 36)
Specification