Methods of inducing regulated pancreatic hormone production in non-pancreatic islet tissues

• US 20040213769A1
• Filed: 05/24/2004
• Published: 10/28/2004
• Est. Priority Date: 06/01/1999
• Status: Active Grant

• Current Assignee: Tel Hashomer Medical Research Infrastructure And Services Ltd.
• Original Assignee: Sarah Ferber
• Inventors: Ferber, Sarah
• Granted Patent: US 8,119,405 B2
• Time in Patent Office: Days
• US Class Current: 424/93.21
• CPC Class Codes: A61K 38/1709 from mammals
• CPC Class Codes: A61K 48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• CPC Class Codes: A61P 1/18 for pancreatic disorders, e.g. pancreatic enzymes
• CPC Class Codes: A61P 3/10 for hyperglycaemia, e.g. antidiabetics
• CPC Class Codes: A61P 5/48 of the pancreatic hormones
• CPC Class Codes: A61P 5/50 for increasing or potentiating the activity of insulin

Abstract

Disclosed are methods and pharmaceutical compositions for inducing pancreatic hormone production.

Claims

1. A method of inducing or enhancing a pancreatic islet cell phenotype or function in a non-pancreatic cell, said method comprising contacting said cell with a compound which increases PDX expression or activity in an amount sufficient to induce or enhance pancreatic islet cell phenotype or function in said cell.

2. The method of claim 1, wherein said PDX is ectopic or endogenous

3. The method of claim 1, wherein said cell is a pluripotent stem cell.

4. The method of claim 1, wherein said cell is a hepatic stem cell.

5. The method of claim 1, wherein said cell is a hepatocyte.

6. The method of claim 1, wherein the cell is provided in vitro.

7. The method of claim 1, wherein the cell is provided ex vivo from a mammalian subject.

8. The method of claim 1, wherein the cell is provided in vivo.

9. The method of claim 1, wherein the compound is a PDX polypeptide, a nucleic acid encoding a PDX polypeptide, or a nucleic acid that increases expression of a nucleic acid that encodes a PDX polypeptide.

10. The method of claim 9, wherein said nucleic acid encoding a PDX polypeptide is a DNA molecule.

11. The method of claim 9, wherein said nucleic acid encoding a PDX polypeptide is present in a plasmid.

12. The method of claim 9, wherein said nucleic acid encoding a PDX polypeptide is present in a viral vector.

13. The method of claim 9, wherein said nucleic acid encoding a PDX polypeptide is encapsulated in a virus.

14. The method of claim 13, wherein said virus is hepatotropic

15. The method of claim 8, wherein said compound is administered by a route selected from the group consisting of intraperitoneal, subcutaneous, nasal, intravenous, intrahepatic, oral and transdermal delivery.