PCR sample preparation holder and method
First Claim
1. An apparatus for preparing samples for Polymerase Chain Reaction analysis comprising:
- a handle having a chamber;
a swab attached to an end of the handle, wherein the swab includes a stabilized reagent in the chamber and a sample tube extending from an end of the chamber;
a wand assembly including a buffer container at an end of a shaft and a stand at another end of the shaft; and
wherein the handle is configured to be attached to the wand assembly.
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Accused Products
Abstract
A simplified Polymerase Chain Reaction (PCR) sample preparation apparatus and method of using a PCR sample preparation for both liquid and dry samples. A portable apparatus comprising a handle removably attached to a wand assembly. The handle includes a cover and a swab with an absorbent material. The wand assembly includes a buffer container for a buffer that is ruptured by a spike when the holder is inserted into the wand assembly. The apparatus may include a waste container removably attached to a tube comprising a plunger, a safety clip to prevent the plunger from depressing, and a port for introduction of a liquid sample. A filter on the waste container collects a specimen of the liquid sample for preparation as described above.
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Citations
32 Claims
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1. An apparatus for preparing samples for Polymerase Chain Reaction analysis comprising:
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a handle having a chamber;
a swab attached to an end of the handle, wherein the swab includes a stabilized reagent in the chamber and a sample tube extending from an end of the chamber;
a wand assembly including a buffer container at an end of a shaft and a stand at another end of the shaft; and
wherein the handle is configured to be attached to the wand assembly. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 16, 17, 18, 19, 20, 21, 22, 23, 24, 26, 27, 28, 29, 30, 32)
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15. An apparatus for preparing liquid samples comprising:
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a waste container, a tube with a port for introduction of a liquid sample and a plunger for pushing the liquid sample into the waste container; and
wherein the tube is removably attached to the waste container.
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25. A biological sampling method for dry and/or processed samples, comprising the following steps:
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providing a handle containing an absorbent material and stabilized reagent and wiping the absorbent material over a test surface; and
providing a wand assembly and inserting the handle into the wand assembly, thereby rupturing a buffer container to enable a buffer to be released through the absorbent material and into a chamber for mixing with the reagent and filling a sample tube.
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31. A biological sampling method for liquid samples comprising the following steps:
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providing a waste container having a plunger and a port, and introducing the liquid sample into the port;
removing a safety clip and depressing the plunger up to a stop on the plunger, to concentrate particles in the liquid sample onto a filter, such that a spike ruptures a liquid container and the plunger pumps a buffer through the filter and into the waste container to wash inhibitors off the particles, and such that a sample collects on the filter;
removing the plunger and discarding the plunger; and
providing a swab and inserting the swab to pick up the sample from the filter in the waste container.
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Specification