Measurement of the cell activity and cell quantity
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Abstract
This invention uses a receptor molecule such as an antibody etc immobilized to a chip for a solid phase, and a sensor such as a pH electrode or an oxygen electrode or a glucose electrode for a search device. After immobilizing specific living cells by a bio-specific recognition reaction (a special immunity bond reaction) and washing the solid phase chip, one can measure the change of pH or oxygen consumption or glucose consumption from the metabolism of the specific living cells in substrate solution. This makes it possible to measure the activity and quantity of living cells specifically, accurately and quickly.
51 Citations
63 Claims
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1-43. -43 (cancelled)
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44. :
- A chip for immobilizing at least one specific living cell or at least one specific living organelle in a sample, comprising;
(a) a surface comprising polyionic layers in an alternating arrangement of at least one polyanion layer and at least one polycation layer substantially parallel to the surface of said chip; and
(b) at least one receptor molecule affixed to said surface and capable of binding a specific molecule on a surface of said at least one specific living cell or organelle. - View Dependent Claims (45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63)
- A chip for immobilizing at least one specific living cell or at least one specific living organelle in a sample, comprising;
Specification