Detection of analytes in fecal samples
First Claim
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1. A method for detecting the presence or absence of a parasitic worm infection in an animal comprising:
- contacting the soluble worm component of a fecal sample from the animal with a first antibody raised against the trichloroacetic acid soluble fraction of a disrupted parasitic worm; and
detecting the presence or absence of an antigen/antibody complex thereby determining the presence or absence of a parasitic worm infection in the animal.
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Abstract
A method for detecting a parasitic worm infection of the digestive tract of a mammal. The method includes detecting the binding of a worm antibody to a worm antigen present in the soluble portion of a fecal sample of infected mammals.
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Citations
21 Claims
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1. A method for detecting the presence or absence of a parasitic worm infection in an animal comprising:
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contacting the soluble worm component of a fecal sample from the animal with a first antibody raised against the trichloroacetic acid soluble fraction of a disrupted parasitic worm; and
detecting the presence or absence of an antigen/antibody complex thereby determining the presence or absence of a parasitic worm infection in the animal. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method for detecting the presence or absence of a parasitic worm antigen in animal feces comprising:
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(a) forming a mixture comprising the soluble portion of a fecal sample and an antibody raised against the trichloroacetic acid soluble fraction of a disrupted parasitic worm that specifically binds a heartworm antigen;
(b) detecting whether the antibody specifically binds an antigen in the fecal sample, thereby determining a the presence of a parasitic worm antigen in feces. - View Dependent Claims (11, 12, 13, 14, 15)
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16. A method for detecting whether the digestive tract of an animal is infected with a parasitic worm comprising:
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immobilizing antibody to heartworm on a solid phase;
adding a soluble portion of a fecal sample from the animal to the solid phase;
detecting the binding of a parasitic worm antigen to the immobilized antibody, thereby detecting whether the animal is infected with a parasitic worm. - View Dependent Claims (17, 18, 19)
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20. A method for detecting the presence or absence of an intestinal parasitic worm infection comprising:
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(a) providing a lateral flow immunoassay device having a solid phase;
(b) providing a first antibody raised against a heartworm antigen;
(c) immobilizing the first antibody on the solid phase of the immunoassay device;
(d) providing a second, labeled antibody;
(e) providing a soluble fraction of a fecal sample;
(f) contacting the soluble fraction of the fecal sample with the second, labeled antibody;
(g) allowing the soluble fraction of the fecal sample and the second, labeled antibody to flow along the immunoassay device towards the immobilized first antibody; and
(h) determining the presence or absence of an intestinal parasite by observing the presence or absence of a signal in the vicinity of the first, immobilized antibody.
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21. A method for detecting the presence of absence of an intestinal parasitic worm infection in an animal comprising:
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(a) running a blood sample from the animal on a heartworm antigen detection device to determine the presence or absence of heartworm antigen in the blood sample; and
(b) if the blood sample is negative for the presence of heartworm antigen, running a fecal sample from the same animal on a parasitic worm antigen detection device to determine the presence or absence of parasitic worm antigen in the fecal sample.
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Specification