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Detection of nucleic acids by type-specific hybrid capture method

  • US 20040214302A1
  • Filed: 04/04/2003
  • Published: 10/28/2004
  • Est. Priority Date: 06/15/2000
  • Status: Active Grant
First Claim
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1. A method of detecting a target nucleic acid comprising:

  • a) hybridizing a single-stranded or partially single-stranded target nucleic acid to a capture sequence probe and a signal sequence probe to form double-stranded hybrids between said probes and the target nucleic acid, wherein the capture sequence probe and the signal sequence probe are capable of hybridizing to non-overlapping regions within the target nucleic acid and not hybridizing to each other;

    b) adding a blocker probe to the hybridization reaction, wherein said blocker probe hybridizes to excess non-hybridized capture sequence probes;

    c) binding the hybrid to a solid phase to form a bound hybrid; and

    d) detecting the bound hybrid.

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